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[物种名称]中WRKY转录因子基因的全基因组分析:对进化特征和萜类化合物合成的洞察

Genome-wide analysis of WRKY transcription factor genes in : An insight into evolutionary characteristics and terpene synthesis.

作者信息

Ren Liping, Wan Wenyang, Yin Dandan, Deng Xianhui, Ma Zongxin, Gao Ting, Cao Xiaohan

机构信息

Key Laboratory of Horticultural Plant Biology of Biological and Food Engineering School, Fuyang Normal University, Fuyang, China.

Horticultural Institute, Fuyang Academy of Agricultural Sciences, Fuyang, China.

出版信息

Front Plant Sci. 2023 Jan 20;13:1063850. doi: 10.3389/fpls.2022.1063850. eCollection 2022.

Abstract

transcription factors (TFs), one of the largest TF families, serve critical roles in the regulation of secondary metabolite production. However, little is known about the expression pattern of genes during the germination and maturation processes of buds. In the present study, the new assembly of the genome was used for the identification of 78 genes, including gene structures, phylogenetic features, chromosomal locations, conserved protein domains, cis-regulatory elements, synteny, and expression profiles. Gene duplication analysis revealed that gene tandem and segmental duplication events drove the expansion of the family, with the latter playing a key role in the creation of new genes. The synteny and evolutionary constraint analyses of the proteins among and several distinct species provided more detailed evidence of gene evolution for . Besides, the expression patterns and co-expression network analysis show may multi-genes co-participate in regulating terpenoid biosynthesis. The findings revealed that potentially play a regulatory role in secondary metabolite synthesis, forming the basis for further functional characterization of genes with the intention of improving .

摘要

转录因子(TFs)是最大的TF家族之一,在次生代谢产物合成的调控中发挥着关键作用。然而,关于芽萌发和成熟过程中基因的表达模式知之甚少。在本研究中,新组装的基因组用于鉴定78个基因,包括基因结构、系统发育特征、染色体位置、保守蛋白结构域、顺式调控元件、共线性和表达谱。基因重复分析表明,基因串联和片段重复事件推动了该家族的扩张,后者在新基因的产生中起关键作用。对该蛋白在某物种和几个不同物种间的共线性和进化约束分析为其基因进化提供了更详细的证据。此外,表达模式和共表达网络分析表明可能有多基因共同参与调节萜类生物合成。研究结果表明该转录因子可能在次生代谢产物合成中起调控作用,为进一步对该基因进行功能表征以改善某状况奠定了基础。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bab0/9895799/1da99e248fe6/fpls-13-1063850-g001.jpg

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