Cupo J F, Rezanka L J, Harpst J A
Department of Biochemistry, School of Medicine, Case Western Reserve University, Cleveland, Ohio 44106.
Anal Biochem. 1987 Jul;164(1):267-70. doi: 10.1016/0003-2697(87)90396-4.
A simple and efficient technique for purifying adenoviral chromatin (nucleoprotein cores) with Sephacryl S-1000 is described. This method is significantly faster than previous methods and gives a higher degree of purity with an increased recovery of the nucleoprotein. In addition, the structural integrity of the cores is maintained.