Moosai R B, Carter M J, Madeley C R
J Clin Pathol. 1984 Dec;37(12):1404-8. doi: 10.1136/jcp.37.12.1404.
A simple and rapid procedure for identifying adenovirus and rotavirus in stool extracts has been developed. The technique is based on polyacrylamide gel electrophoresis of the virus nucleic acid, but sample preparation is straightforward and does not entail phenol extraction or the use of a radioactive label. Furthermore, processing is not influenced by the amount of specimen obtained and is thus suitable for application as a batch testing method. This approach removes the need for specific antisera, which are not readily available since most of these viruses cannot be grown using routine tissue culture procedures. Trials in this laboratory have indicated that the technique is of comparable sensitivity to electron microscopy.
已开发出一种简单快速的方法来鉴定粪便提取物中的腺病毒和轮状病毒。该技术基于病毒核酸的聚丙烯酰胺凝胶电泳，但样品制备简单，无需酚提取或使用放射性标记。此外，处理过程不受所获标本量的影响，因此适合作为批量检测方法应用。这种方法无需使用特异性抗血清，因为大多数这些病毒无法通过常规组织培养程序培养，所以特异性抗血清不易获得。本实验室的试验表明，该技术与电子显微镜的灵敏度相当。
