Albertsson P A, Hultin E
Department of Biochemistry, University of Lund, Sweden.
Anal Biochem. 1987 Oct;166(1):218-23. doi: 10.1016/0003-2697(87)90567-7.
The single withdrawal technique, here called elution countercurrent distribution, was applied for the first time on aqueous two-phase systems. The modifications necessary for elution countercurrent distribution of a thin-layer countercurrent distribution apparatus are described. The results from a test run with sulfuric acid showed good agreement between the experimental and the theoretical curves for elution countercurrent distribution. Thylakoid membrane vesicles from spinach chloroplasts were subjected to the elution countercurrent distribution. A gradient of NaCl in the eluting upper phase was used to elute the different fractions in order of their affinity for the moving upper phase. Inside-out thylakoid vesicles were resolved into two major and several minor fractions having different chlorophyll a/b ratios. Sonicated inside-out thylacoid vesicles were resolved into at least five different fractions with different chlorophyll a/b ratios. An increased resolution is obtained with elution countercurrent distribution compared to the fundamental process.
单级萃取技术，这里称为洗脱逆流分配，首次应用于双水相体系。描述了薄层逆流分配装置进行洗脱逆流分配所需的改进。硫酸试验运行的结果表明，洗脱逆流分配的实验曲线与理论曲线吻合良好。对菠菜叶绿体的类囊体膜囊泡进行洗脱逆流分配。使用洗脱上层相中NaCl的梯度，按照它们对移动上层相的亲和力顺序洗脱不同的组分。外翻的类囊体囊泡被分离成两个主要组分和几个次要组分，它们具有不同的叶绿素a/b比值。超声处理的外翻类囊体囊泡被分离成至少五个具有不同叶绿素a/b比值的不同组分。与基本方法相比，洗脱逆流分配获得了更高的分辨率。
