Titovets E
Experimental Department, Byelorussian State Research Institute of Neurology, Neurosurgery, and Physiotherapy, Minsk, Union of Soviet Socialist Republics.
Anal Biochem. 1987 Oct;166(1):79-82. doi: 10.1016/0003-2697(87)90548-3.
A membrane-diffusion cell for oxygen uptake measurements in an open system is described. Polarographic oxygen measurements with a Clark electrode are combined with simultaneous pH and absorbance or fluorescence determinations. Examples of its use are given and discussed, including experiments on mitochondrial respiration. Advantages of this cell are analyzed and comparisons are made with other open-system respirographs.
本文描述了一种用于开放系统中氧摄取量测量的膜扩散池。使用Clark电极进行极谱氧测量,并同时进行pH值、吸光度或荧光测定。文中给出并讨论了该扩散池的使用示例,包括线粒体呼吸实验。分析了该扩散池的优点,并与其他开放系统呼吸描记器进行了比较。