Tomov T C
J Biochem Biophys Methods. 1986 Aug;13(1):29-38. doi: 10.1016/0165-022x(86)90005-9.
Added to mitochondrial suspension, pyronin G changes the intensity of its fluorescence depending on the membrane potential (energy state) of the mitochondria. The mechanism of this effect is studied and a dependence is obtained between the membrane potential and the fluorescence intensity. This permits quantitative determination of the membrane potential by the changes in the fluorescence of the suspension. A method is proposed for measuring the membrane potential of vesicles in the -120 to -220 mV interval.
将派洛宁G添加到线粒体悬浮液中，它会根据线粒体的膜电位（能量状态）改变其荧光强度。研究了这种效应的机制，并得出了膜电位与荧光强度之间的关系。这使得可以通过悬浮液荧光的变化对膜电位进行定量测定。提出了一种测量-120至-220 mV区间内囊泡膜电位的方法。
