Jaroensan Jedsada, Khiaophong Wannipha, Kachangoon Rawikan, Vichapong Jitlada
Creative Chemistry and Innovation Research Unit, Department of Chemistry and Center of Excellence for Innovation in Chemistry, Faculty of Science, Mahasarakham University Maha Sarakham 44150 Thailand
Multidisciplinary Research Unit of Pure and Applied Chemistry (MRUPAC), Department of Chemistry and Center of Excellent for Innovation in Chemistry, Faculty of Science, Mahasarakham University Maha Sarakham 44150 Thailand.
RSC Adv. 2023 Jan 31;13(7):4195-4201. doi: 10.1039/d2ra06560f.
An enrichment method, namely popping candy-generated CO and sugaring-out-assisted supramolecular solvent-based microextraction (PGS-SUPRA), was investigated for the determination of triazole fungicide residues in water, honey and soy milk samples. The extraction process was carried out by adding popping candies into a centrifuge tube. Consequently, rapid dispersion and mass transfer of extractants can be achieved without using dispersants and auxiliary devices, and therefore, the extraction efficiency increased. The extraction parameters affecting the efficiency of the developed method were investigated. The presented method was then analysed by high-performance liquid chromatography. Under the selected condition, the wide linearity of triazole fungicides after preconcentration by the proposed microextraction method ranged from 30 to 1000 μg L for triadimefon and from 90 to 1000 μg L for myclobutanil, tebuconazole and hexaconazole, with a coefficient for determination ( ) greater than 0.992. The limits of detection (LODs) and limits of quantitation (LOQs) were in the range of 10-30 μg L and 30-90 μg L, respectively. The precisions were assessed from the relative standard deviations (RSDs) of the retention time and peak area obtained from intra- ( = 3) and inter-day ( = 3 × 5) experiments, and were greater than 1.66% and 13.52%, respectively. Moreover, the proposed method provided high enhancement factors (EnFs) ranging from 14 to 51 folds. This technique has been prosperously applied for the extraction of fungicide residues in water, honey and soy milk samples with a recovery within the range of 60-114%. Overall, the developed method was found to be advantageous as compared with other sample preparation methods.
研究了一种富集方法，即爆珠糖产生一氧化碳和析糖辅助超分子溶剂微萃取法（PGS-SUPRA），用于测定水、蜂蜜和豆浆样品中的三唑类杀菌剂残留。萃取过程是通过将爆珠糖加入离心管中进行的。因此，无需使用分散剂和辅助装置就能实现萃取剂的快速分散和传质，从而提高了萃取效率。研究了影响该方法效率的萃取参数。然后用高效液相色谱法对该方法进行分析。在选定的条件下，采用所提出的微萃取方法预富集后，三唑类杀菌剂的线性范围较宽，多菌灵为30至1000 μg/L，腈菌唑、戊唑醇和己唑醇为90至1000 μg/L，测定系数（ ）大于0.992。检测限（LODs）和定量限（LOQs）分别在10-30 μg/L和30-90 μg/L范围内。通过日内（ = 3）和日间（ = 3×5）实验获得的保留时间和峰面积的相对标准偏差（RSDs）评估精密度，分别大于1.66%和13.52%。此外，所提出的方法提供了14至51倍的高富集因子（EnFs）。该技术已成功应用于水、蜂蜜和豆浆样品中杀菌剂残留的萃取，回收率在60-114%范围内。总体而言，与其他样品制备方法相比，所开发的方法具有优势。
