Basic Science Laboratory, Faculty of Stomatology, San Luis Potosí University, San Luis Potosi, Mexico.
Biochemical and Microbiology Laboratory, Faculty of Stomatology, San Luis Potosí University, San Luis Potosi, Mexico.
Biomed Mater Eng. 2023;34(4):357-373. doi: 10.3233/BME-222513.
Acrylic resins used in dental and biomedical applications do not have antimicrobial properties, their surface is susceptible to colonization of microorganisms.
The aim of this study was to evaluate the antibiofilm properties of silver nanoparticles (AgNPs) deposited in a polymethyl methacrylate (PMMA) surface against a Staphylococcus aureus biofilm.
The PMMA was impregnated with AgNPs by using the in-situ polymerization method. To determine the solubility of the incorporated silver (Ag+) atomic absorption spectrophotometry was used (AAS) at 24 h, 48 h, 7 days, and 30 days. Thirty specimens of PMMA with AgNPs and without NP (control group) were assembled in the CDC Biofilm Bioreactor system with a cell suspension of S. aureus. The specimens were removed at 6, 12, 24, 48, and 72 h to determine the viability profile and quantify the Arbitrary Fluorescence Units (AFU).
The AgNPs showed an irregular and quasispherical shape with an average size of 25 nm. AAS analysis demonstrated a low solubility of Ag+. The formation of the S. aureus biofilm increased as the evaluation periods continued up to 72 h. The experimental group showed poor growth, and a decrease in the intensity of the fluorescence demonstrated a statistically significant inhibition of the formation of the biofilm (P < 0.05) in relation to the control group at 6, 12, 24, 48, and 72 h.
AgNPs incorporated into PMMA decreased the growth and maturation of S. aureus biofilm.
用于牙科和生物医学应用的丙烯酸树脂没有抗菌性能，其表面容易被微生物定植。
本研究旨在评估沉积在聚甲基丙烯酸甲酯（PMMA）表面的纳米银颗粒（AgNPs）对金黄色葡萄球菌生物膜的抗生物膜特性。
采用原位聚合法将 PMMA 浸渍在 AgNPs 中。为了确定结合银的溶解度（Ag+），使用原子吸收分光光度法（AAS）在 24 小时、48 小时、7 天和 30 天进行测量。将 30 个带有 AgNPs 和无 NP（对照组）的 PMMA 标本组装在 CDC 生物膜生物反应器系统中，其中含有金黄色葡萄球菌的细胞悬浮液。在 6、12、24、48 和 72 小时时取出标本，以确定生存曲线并量化任意荧光单位（AFU）。
AgNPs 呈不规则的准球形，平均粒径为 25nm。AAS 分析表明 Ag+的溶解度较低。随着评估时间的延长，金黄色葡萄球菌生物膜的形成增加，持续至 72 小时。实验组的生长情况较差，荧光强度的降低表明与对照组相比，在 6、12、24、48 和 72 小时时，生物膜的形成受到了统计学上显著的抑制（P<0.05）。
掺入 PMMA 的 AgNPs 降低了金黄色葡萄球菌生物膜的生长和成熟。
