Beijing Laboratory for Food Quality and Safety, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China.
Beijing Laboratory for Food Quality and Safety, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China.
Int J Biol Macromol. 2022 Jan 15;195:515-522. doi: 10.1016/j.ijbiomac.2021.12.031. Epub 2021 Dec 14.
Multi-drug resistance and biofilm formation are a growing problem in the treatment of Staphylococcus aureus contamination. Advances in nanotechnology allow the synthesis of metal nanoparticles that can be assembled into complex architectures for controlling bacterial growth. This study aims to investigate the ultrasonic-assisted green synthesis of silver nanoparticles (AgNPs) by tannic acid (TA) and sodium alginate (Na-Alg) as the reducing and stabilizing agents, respectively, and evaluation of their antibacterial and antibiofilm activities. The UV-Vis spectroscopy and transmission electron microscopy (TEM), energy dispersive spectroscopy (EDS), zetasizer, FT-IR spectroscopy, and X-ray diffraction (XRD) studies showed that the optimum produces were spherical, stable, and monodispersed AgNPs with an average size of particle sizes of 18.52 ± 0.07 nm. The antibacterial and antibiofilm activities of the AgNPs loaded TA/Na-Alg constructs against S. aureus ATCC 6538 were investigated. The minimum inhibitory concentration (MIC) of the AgNPs was 31.25 μg/mL. After exposure to the AgNPs, planktonic S. aureus showed irreversible cell membrane damage, decreased cell viability, and changes in cellular morphology. In addition, the AgNps significantly inhibited S. aureus biofilm formation at 1/32 MIC. The biofilm elimination rate was 58.87% after exposure to MIC AgNPs. The results suggested that the development of AgNPs loaded TA/Na-Alg constructs with biomedical potentialities obtained through a simple, green, and cost-effective approach, may be suitable for the formulation of a new strategy for combating S. aureus.
多药耐药性和生物膜形成是金黄色葡萄球菌污染治疗中的一个日益严重的问题。纳米技术的进步允许合成金属纳米粒子，这些粒子可以组装成复杂的结构来控制细菌的生长。本研究旨在通过单宁酸（TA）和海藻酸钠（Na-Alg）分别作为还原剂和稳定剂，研究超声辅助绿色合成银纳米粒子（AgNPs），并评价其抗菌和抗生物膜活性。紫外-可见光谱和透射电子显微镜（TEM）、能量色散光谱（EDS）、Zetasizer、傅里叶变换红外光谱（FT-IR）和 X 射线衍射（XRD）研究表明，最佳产物为球形、稳定、单分散的 AgNPs，平均粒径为 18.52±0.07nm。研究了负载 TA/Na-Alg 构建体的 AgNPs 对金黄色葡萄球菌 ATCC 6538 的抗菌和抗生物膜活性。AgNPs 的最小抑菌浓度（MIC）为 31.25μg/ml。暴露于 AgNPs 后，浮游状态的金黄色葡萄球菌表现出不可逆的细胞膜损伤、细胞活力降低和细胞形态变化。此外，AgNps 可显著抑制 1/32MIC 时金黄色葡萄球菌生物膜的形成。暴露于 MIC AgNPs 后，生物膜消除率为 58.87%。结果表明，通过简单、绿色、经济有效的方法获得具有生物医学潜力的负载 TA/Na-Alg 构建体的 AgNPs 的开发，可能适合制定对抗金黄色葡萄球菌的新策略。
