CDH17 阳性循环肿瘤细胞对结直肠癌患者的诊断意义。

The diagnostic significance of CDH17-positive circulating tumor cells in patients with colorectal cancer.

机构信息

Department of Applied Biology & Chemical Technology, The Hong Kong Polytechnic University, Hong Kong, Hong Kong Special Administrative Region, China.

Department of Pathology, Kiang Wu Hospital, Macao, Macau Special Administrative Region, China.

出版信息

Expert Rev Mol Diagn. 2023 Feb;23(2):171-179. doi: 10.1080/14737159.2023.2176223. Epub 2023 Feb 9.

DOI:10.1080/14737159.2023.2176223

PMID:36744385

Abstract

BACKGROUND

Colorectal cancer (CRC) is the second leading cause of cancer deaths in Hong Kong. We tested the hypothesis that circulating tumor cell (CTC) analysis by ARB101 antibody could be used as a tool for CRC detection, progression, and therapy response.

RESEARCH METHODS

ARB101 antibody was used for investigation of CDH17 expression in formalin-fixed, paraffin-embedded (FFPE) tissue sections and circulating tumor cells (CTCs) of CRC patients.

RESULTS

Using ARB101, highest sensitivity was observed in 98/100 (98%) colorectal cancer tissue compared to 72/100 gastric cancer (72%) and 27/32 pancreatic cancer (84%). Immunoreactivity of CDH17 was significantly higher in distant metastatic (tumor-node-metastasis [TNM] stage IV) than non-distant metastatic (TNM stage I to III) CRC. ARB101 antibody also manifested the higher sensitivity than c-erbB2 (8%) and epidermal growth factor receptor (EGFR)-targeting antibodies (37%) with the significance (p < 0.0001). ARB101 positive CTCs were detected in 64/83 (77%) TNM stage I to IV CRC patients. Furthermore, ARB101 positive CTCs detected in TNM stage I to III CRC patients before and after surgical operation are statistically significant (p < 0.0001).

CONCLUSIONS

CTC detection by ARB101 antibody could serve as a potential non-invasive approach for CRC detection, progression, and monitoring of treatment response.

摘要

背景

结直肠癌（CRC）是香港癌症死亡的第二大主要原因。我们检验了这样一个假设，即通过 ARB101 抗体进行循环肿瘤细胞（CTC）分析可作为 CRC 检测、进展和治疗反应的工具。

研究方法

使用 ARB101 抗体检测 CRC 患者福尔马林固定石蜡包埋（FFPE）组织切片和循环肿瘤细胞（CTC）中 CDH17 的表达。

结果

使用 ARB101 抗体，在 100 例结直肠癌组织中观察到最高的敏感性，为 98/100（98%），而在 100 例胃癌和 32 例胰腺癌中分别为 72/100（72%）和 27/32（84%）。在远处转移（肿瘤-淋巴结-转移 [TNM] 第 IV 期）CRC 中，CDH17 的免疫反应性明显高于非远处转移（TNM 第 I 至 III 期）CRC。与 c-erbB2（8%）和表皮生长因子受体（EGFR）靶向抗体（37%）相比，ARB101 抗体的敏感性更高，差异具有统计学意义（p<0.0001）。在 83 例 TNM Ⅰ至Ⅳ期 CRC 患者中，检测到 64/83（77%）患者存在 ARB101 阳性 CTC。此外，在手术前后 TNM Ⅰ至Ⅲ期 CRC 患者中检测到 ARB101 阳性 CTC，差异具有统计学意义（p<0.0001）。