Zhang Yixin, Tan Jiawei, Chen Yiyun
State Key Laboratory of Bioorganic and Natural Products Chemistry Centre of Excellence in Molecular Synthesis, Shanghai Institute of Organic Chemistry, University of Chinese Academy of Sciences, Chinese Academy of Sciences, 345 Lingling Road, Shanghai 200032, P. R. China.
School of Physical Science and Technology ShanghaiTech University, 100 Haike Road, Shanghai 201210, P. R. China.
Chem Commun (Camb). 2023 Feb 23;59(17):2413-2420. doi: 10.1039/d2cc06987c.
Chemical labeling of proteins in live cells helps to probe their native functions in biological systems. Aryl azides are chemically inert under physiological conditions, but they are activated by certain external stimuli. Recently, photocatalytic live-cell applications of aryl azides by visible light irradiation have become a burgeoning new field in chemical biology. In this Feature Article, we focus on the recent progress of protein labeling in live cells with aryl azides induced by visible-light irradiation. Light irradiation activates aryl azides to generate highly reactive intermediates, which enables protein labeling for protein functionalization, crosslinking, and profiling. The activation mechanism of aryl azides by light irradiation is categorized as photolysis, energy-transfer, and electron-transfer. The extracellular and intracellular protein labeling applications in live cells with aryl azides induced by visible light are discussed, including recent advances in red-light-induced extracellular protein labeling.
对活细胞中的蛋白质进行化学标记有助于探究其在生物系统中的天然功能。芳基叠氮化物在生理条件下化学性质不活泼，但可被某些外部刺激激活。最近，通过可见光照射实现芳基叠氮化物在光催化活细胞中的应用已成为化学生物学中一个新兴的新领域。在这篇专题文章中，我们重点关注可见光照射诱导的芳基叠氮化物在活细胞中进行蛋白质标记的最新进展。光照射激活芳基叠氮化物以产生高活性中间体，从而实现用于蛋白质功能化、交联和分析的蛋白质标记。芳基叠氮化物通过光照射的激活机制分为光解、能量转移和电子转移。讨论了可见光诱导的芳基叠氮化物在活细胞中的细胞外和细胞内蛋白质标记应用，包括红光诱导的细胞外蛋白质标记的最新进展。
