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银杏雌雄株的 LAMP 鉴别。

Distinction of Male and Female Trees of Ginkgo biloba Using LAMP.

机构信息

Department of Microbiology, Chungbuk National University, Seowon-Gu, Cheongju, 28644, South Korea.

出版信息

Mol Biotechnol. 2023 Oct;65(10):1693-1703. doi: 10.1007/s12033-023-00673-7. Epub 2023 Feb 6.

DOI:10.1007/s12033-023-00673-7
PMID:36745281
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9901403/
Abstract

Ginkgo biloba is utilized as food, medicine, wood, and street trees among other things. The objective of this study was to develop a loop-mediated isothermal amplification (LAMP) assay for gender distinction of G. biloba. Male-specific SCAR gene can be utilized to identify G. biloba gender using LAMP. The optimized LAMP conditions, temperature 60 °C, 2-mM MgSO, and [F3/B3]:[FIP/BIP] primer ratio of 1:4 were selected as final conditions. The G. biloba SCAR LAMP displayed a sensitivity of 10 ng when amplified by concentration under the optimum conditions. Additionally, it demonstrated a particular response in male with SYBR Green I in LAMP analysis that can be a more powerful tool for field and scale-up applications. Our work represents a first attempt to identify G. biloba gender using LAMP and offers an efficient and reliable tool for roadside landscaping.

摘要

银杏被广泛应用于食品、药品、木材和街道绿化等领域。本研究旨在开发一种环介导等温扩增（LAMP）技术，用于区分银杏的性别。利用雄性特异性 SCAR 基因，可以通过 LAMP 鉴定银杏的性别。优化后的 LAMP 条件为 60°C、2-mM MgSO4 和 [F3/B3]：[FIP/BIP] 引物比为 1:4。在最佳条件下，SCAR LAMP 的灵敏度为 10ng。此外，在 LAMP 分析中，它对雄性银杏表现出特异性反应，这使其成为现场和规模化应用的更有力工具。我们的工作首次尝试使用 LAMP 技术来识别银杏的性别，为道路景观提供了一种高效可靠的工具。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a9b8/9901403/23f4545d8eea/12033_2023_673_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a9b8/9901403/8fd3234adc81/12033_2023_673_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a9b8/9901403/cace9f082e34/12033_2023_673_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a9b8/9901403/676aee84ec82/12033_2023_673_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a9b8/9901403/588f1f47f164/12033_2023_673_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a9b8/9901403/5e21df62ee0d/12033_2023_673_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a9b8/9901403/23f4545d8eea/12033_2023_673_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a9b8/9901403/8fd3234adc81/12033_2023_673_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a9b8/9901403/cace9f082e34/12033_2023_673_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a9b8/9901403/676aee84ec82/12033_2023_673_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a9b8/9901403/588f1f47f164/12033_2023_673_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a9b8/9901403/5e21df62ee0d/12033_2023_673_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a9b8/9901403/23f4545d8eea/12033_2023_673_Fig6_HTML.jpg

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