Herbal Medicine Research Division, Korea Institute of Oriental Medicine, Daejeon 305-811, Korea.
Phyzen Genomics Institute, Seongnam 13558, Korea.
Molecules. 2018 Jul 17;23(7):1748. doi: 10.3390/molecules23071748.
Dried roots of (Caprifoliaceae) are used as important traditional herbal medicines in Korea. However, the roots are often used as a mixture or contaminated with in Korean herbal markets. Furthermore, the dried roots of (Lamiaceae) are used indiscriminately with those of , with the confusing Korean names of Sok-Dan and Han-Sok-Dan for and , respectively. Although and are important herbal medicines, the molecular marker and genomic information available for these species are limited. In this study, we analysed DNA barcodes to distinguish among , , and and sequenced the chloroplast (CP) genomes of and . The CP genomes of and were 160,530 and 160,371 bp in length, respectively, and were highly divergent from those of the other Caprifoliaceae species. Phylogenetic analysis revealed a monophyletic group within Caprifoliaceae. We also developed a novel sequence characterised amplified region (SCAR) markers to distinguish among , , and . Our results provide important taxonomic, phylogenetic, and evolutionary information on the species. The SCAR markers developed here will be useful for the authentication of herbal medicines.
（忍冬科）的干燥根被用作韩国重要的传统草药。然而，这些根经常被用作混合物或与（唇形科）的根混合污染。此外，（唇形科）的干燥根被与混淆的韩国名字 Sok-Dan 和 Han-Sok-Dan 与 和 一起不加区分地使用，分别为 和 。尽管 和 是重要的草药，但这些物种的分子标记和基因组信息有限。在这项研究中，我们分析了 DNA 条形码以区分 、 和 ，并对 和 的叶绿体 (CP) 基因组进行了测序。 和 的 CP 基因组分别长 160,530 和 160,371bp，与其他忍冬科物种高度不同。系统发育分析显示在忍冬科内有一个单系群。我们还开发了一种新的序列特征扩增区域 (SCAR) 标记来区分 、 和 。我们的结果为 物种提供了重要的分类学、系统发育和进化信息。这里开发的 SCAR 标记将有助于草药的鉴定。
