Roubinet F, Béral M M, Legac E, Gariepy P, Blanc C, Cambon-Thomsen A, Debré P
Service commun d'Analyse et de Tri Cellulaire, Universitaire Paul Sabatier, CHJ Purpan, Toulouse.
Ann Biol Clin (Paris). 1987;45(4):423-9.
This is a report on our experience with the EPICS C (Coultronics) cytometric flux apparatus, a screening cell analyzer, employing a laser ray (2 or 5 watts); we obtained good results to analyze immunologically-tagged mononuclear blood cells with or without prior separation: for rhythm, repeatability, and contamination. The EPICS C machine proved to be effective to study the cell cycle using lymphoblastic cells, epithelial cells and cells from a breast cancer. Several screening trials were carried out with fluorescent ball bearings of various sizes; the quality of screening (purity and yield) appear optimal at a speed ranging from 500 to 1,000 bearings per second, using three parameters: the logarithm of green fluorescence, the integral function of green fluorescence, the diffraction of light to small angles. Thus, if results obtained for this analysis are entirely satisfactory, the screening function remains limited because it is slow.
这是一份关于我们使用EPICS C（库尔特电子公司）细胞计数通量仪的经验报告，这是一种采用激光束（2瓦或5瓦）的筛选细胞分析仪；我们在分析有或没有预先分离的免疫标记单核血细胞方面取得了良好结果，涉及节奏、重复性和污染方面。事实证明，EPICS C机器在使用淋巴细胞、上皮细胞和乳腺癌细胞研究细胞周期方面是有效的。使用各种尺寸的荧光滚珠轴承进行了几次筛选试验；在每秒500至1000个滚珠轴承的速度下，使用三个参数：绿色荧光的对数、绿色荧光的积分函数、小角度光衍射，筛选质量（纯度和产量）似乎最佳。因此，如果该分析获得的结果完全令人满意，筛选功能仍然有限，因为它速度较慢。
