Leif R C, Cayer M L, Dailey W, Stribling T, Gordon K
Coulter Corporation, Miami, Florida, USA.
Cytometry. 1995 Jun 1;20(2):185-90. doi: 10.1002/cyto.990200212.
Fused silicon dioxide, multiparameter flow transducers with 50 microns internal square cross section and approximately 60 microns length can simultaneously measure DC and RF impedance as well as fluorescence and multiple-angle light scattering. A spherical version of such a transducer was mounted in an EPICS CVA flow-cell housing and was installed on a research prototype equipped with an argon-ion laser. The signal that was produced by the spherical transducer with EPICS DNA-Check beads was 1.73 times greater than that produced with the standard cylindrical flow cell. Similarly, with EPICS Immuno-Brite beads, the average ratio was 1.96. The Coulter impedance and light-scattering measurements were similar to those produced with the conventional cylindrical outside flow cell, although the internal cross section of the sphere was square and that of the cylinder was circular. The theoretical arguments of Leif and Wells have been demonstrated to be correct. At present, monolithic, spherical fused-silica transducers are the optimal design for combined electrooptical, multiparameter flow cytometry analyzers.
熔融二氧化硅制成的内部方形横截面为50微米、长度约为60微米的多参数流动传感器，能够同时测量直流和射频阻抗以及荧光和多角度光散射。这种传感器的球形版本安装在EPICS CVA流动池外壳中，并安装在配备氩离子激光器的研究原型上。使用EPICS DNA检测珠时，球形传感器产生的信号比标准圆柱形流动池产生的信号大1.73倍。同样，使用EPICS免疫亮珠时，平均比率为1.96。库尔特阻抗和光散射测量结果与传统圆柱形外部流动池的测量结果相似，尽管球体的内部横截面是方形的，而圆柱体的是圆形的。利夫和韦尔斯的理论观点已被证明是正确的。目前，单片球形熔融石英传感器是组合式电光多参数流式细胞仪分析仪的最佳设计。
