*Ayca Sarialioglu Gungor, Department of Restorative Dentistry, Faculty of Dentistry, Bezmialem Vakif University, Istanbul, Turkey.
Ezgi Durmus, Department of Medical Biochemistry, Faculty of Medicine, Bezmialem Vakif University, Istanbul, Turkey.
Oper Dent. 2023 May 1;48(3):317-328. doi: 10.2341/22-023-L.
The objective of this study was to assess the effect of bioactive pulp-capping materials on human dental pulp stem cell (hDPSC) behavior in terms of cell viability and bioactivity via mineralization potential.
Nanoparticles of 58S5 bioactive glass (nBG) powder were elaborated by a sol-gel process. Primer hDPSCs were cultured with experimental nBG, Biodentine, TheraCal LC, and ProRoot mineral trioxide aggregate (MTA) extracts. Cell viability was measured for 1, 3, and 7 days by water-soluble tetrazolium salts (WST-1) assay. Expression of mineralization-related marker genes (dentin sialophosphoprotein [DSPP] and osteocalcin [OCN]) was quantified by a real-time polymerase chain reaction. Detection of DSPP protein expression in hDPSCs was also assessed by western blotting. Alizarin red staining was used to detect the formation of mineralized nodules, and alkaline phosphatase (ALP) activity was quantified by a photometric method (days 7 and 14). All data were statistically analyzed with a one-way analysis of variance (ANOVA) and Tukey's post-hoc test (p<0.05).
The cell viability of hDPSCs in all groups decreased except for nBG, and the lowest cell viability was determined in TheraCal LC at all incubation times. nBG and MTA showed significantly higher ALP activity than the control group. The tested materials elevated the calcium nodule form of hDPSCs except for TheraCal LC. The highest DSPP expression was seen in nBG for both incubation times.
nBG promotes differentiation and mineralization of hDPSCs at a higher rate than other bioactive pulp-capping materials tested.
本研究旨在通过矿化潜力评估生物活性盖髓材料对人牙髓干细胞（hDPSC）行为的影响，包括细胞活力和生物活性。
通过溶胶-凝胶工艺制备 58S5 生物活性玻璃（nBG）纳米颗粒。原代 hDPSC 用实验用 nBG、Biodentine、TheraCal LC 和 ProRoot 矿化三氧化物聚合体（MTA）提取物培养。通过水溶性噻唑盐（WST-1）测定法在第 1、3 和 7 天测量细胞活力。通过实时聚合酶链反应定量测定矿化相关标记基因（牙本质涎磷蛋白[DSPP]和骨钙素[OCN]）的表达。通过 Western blot 检测 hDPSC 中 DSPP 蛋白表达。使用茜素红染色检测矿化结节的形成，并通过比色法（第 7 天和第 14 天）定量测定碱性磷酸酶（ALP）活性。使用单因素方差分析（ANOVA）和 Tukey 事后检验对所有数据进行统计学分析（p<0.05）。
除 nBG 组外，所有组的 hDPSC 细胞活力均下降，在所有孵育时间内 TheraCal LC 组的细胞活力最低。nBG 和 MTA 组的 ALP 活性明显高于对照组。除 TheraCal LC 组外，所有测试材料均能促进 hDPSC 钙结节的形成。在两个孵育时间内，nBG 组的 DSPP 表达最高。
nBG 比其他测试的生物活性盖髓材料更能促进 hDPSC 的分化和矿化。
