Department of Pediatric Dentistry, Faculty of Dentistry, Marmara University, Istanbul, Turkey.
Department of Genetics and Bioengineering, Faculty of Engineering, Yeditepe University, Istanbul, Turkey.
Int Endod J. 2018 Jul;51(7):758-766. doi: 10.1111/iej.12747. Epub 2017 Feb 22.
To investigate the effect of accelerated-set mineral trioxide aggregate (MTA) on the proliferation and odontoblastic differentiation of human dental pulp cell niches (DPSC).
ProRoot White MTA (WMTA; Dentsply Tulsa Dental, Johnson City, TN, USA) was mixed with various additives, which included distilled water, 2.5% disodium hydrogen phosphate (Na HPO ; Merck, Darmstadt, Germany) and 5% calcium chloride (CaCl ; Merck). DPSC niches extracted from third molars were cultured directly on MTA in the culture medium. Cell viability was evaluated by 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4- sulphophenyl)-2H-tetrazolium (MTS) assay. Cell growth and expression of odontoblastic differentiation markers (dentine sialophosphoprotein (DSPP) and collagen type 1 (COL1)) were determined using Real-Time Polymerase Chain Reaction analysis. Osteo-/odontogenic differentiation of DPSC niches was evaluated by measurement of alkaline phosphatase activity (ALP). Calcium deposition was assessed using von Kossa staining. The results were analysed statistically using Mann-Whitney tests and Kruskal-Wallis tests.
MTA mixed with 5% CaCl and 2.5% Na HPO exhibited optimal cell viability (P < 0.05) compared to MTA mixed with distilled water. MTA mixed with 5% CaCl and 2.5% Na HPO significantly increased ALP activity (P < 0.05), significantly promoted mineralization nodule formation (P < 0.05) and significantly enhanced the mRNA expression level of the osteogenic/odontogenic markers (P < 0.05; DSPP and COL1) compared with MTA mixed with distilled water.
MTA mixed with 5% CaCl and 2.5% Na HPO was biocompatible with dental pulp stem cell niches. Accelerated-set MTA promoted better differentiation in DPSC niches than conventional MTA. The accelerators could provide an alternative to MTA mixed with distilled water.
研究加速凝固型矿化三氧化物凝聚体（MTA）对人牙髓细胞龛（DPSC）增殖和成牙本质分化的影响。
ProRoot White MTA（WMTA；登士柏天津牙科有限公司，美国田纳西州约翰逊市）与不同添加剂混合，包括蒸馏水、2.5% 磷酸氢二钠（NaHPO4；默克公司，德国达姆施塔特）和 5% 氯化钙（CaCl2；默克公司）。从第三磨牙中提取的 DPSC 巢直接培养在培养基中的 MTA 上。通过 3-(4,5-二甲基噻唑-2-基)-5-(3-羧甲氧基苯基)-2-(4-磺苯基)-2H-四唑（MTS）法评估细胞活力。通过实时聚合酶链反应分析测定细胞生长和牙本质分化标志物（牙本质涎磷蛋白（DSPP）和胶原蛋白 1（COL1））的表达。通过碱性磷酸酶活性（ALP）测定评估 DPSC 巢的成骨/成牙本质分化。通过 von Kossa 染色评估钙沉积。使用曼-惠特尼检验和克鲁斯卡尔-沃利斯检验对结果进行统计学分析。
与蒸馏水混合的 MTA 相比，与 5%CaCl2 和 2.5%NaHPO4 混合的 MTA 表现出最佳的细胞活力（P<0.05）。与蒸馏水混合的 MTA 相比，与 5%CaCl2 和 2.5%NaHPO4 混合的 MTA 显著增加了 ALP 活性（P<0.05），显著促进了矿化结节的形成（P<0.05），并显著增强了成骨/成牙本质标志物的 mRNA 表达水平（P<0.05；DSPP 和 COL1）。
与蒸馏水混合的 MTA 与牙髓干细胞巢具有生物相容性。加速凝固型 MTA 促进了 DPSC 巢更好的分化，优于传统 MTA。加速剂可以为与蒸馏水混合的 MTA 提供替代方案。
