含5-溴尿嘧啶的DNA中单个光化学事件导致的双链断裂。
Double-strand breaks from single photochemical events in DNA containing 5-bromouracil.
作者信息
Krasin F, Hutchinson F
出版信息
Biophys J. 1978 Dec;24(3):645-56. doi: 10.1016/S0006-3495(78)85410-1.
Abstract
Ultraviolet irradiation of Escherichia coli cells with a low level of 5-bromouracil incorporated produces DNA double-strand breaks by single photochemical events, one such break per 100 single-strand breaks, the latter assayed in alkali-denatured DNA. About 2.5--4 double-strand breaks are produced per "lethal hit," compared with about 6 double-strand breaks per lethal hit induced by gamma rays. These results are consistent with the hypothesis that an unrepaired DNA double-strand break is a major lethal event in both cases. The increase in sensitivity to ultraviolet (measured by colony-forming ability) seems linear in the number of bromouracils incorporated (0--20% of the thymines), and the linear relationship is much the same for incorporation in one or in both strands of the DNA double helix.
摘要
用低水平掺入5-溴尿嘧啶的大肠杆菌细胞进行紫外线照射，通过单个光化学事件产生DNA双链断裂，每100个单链断裂产生一个这样的双链断裂，后者在碱变性DNA中检测。每“致死剂量”产生约2.5 - 4个双链断裂，而γ射线诱导的每致死剂量约产生6个双链断裂。这些结果与以下假设一致：未修复的DNA双链断裂在两种情况下都是主要的致死事件。对紫外线的敏感性增加（通过集落形成能力测量）似乎与掺入的溴尿嘧啶数量（胸腺嘧啶的0 - 20%）呈线性关系，并且对于DNA双螺旋的一条链或两条链中的掺入，线性关系大致相同。
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