Marušič Maja, Toplishek Maria, Plavec Janez
Slovenian NMR Center, National Institute of Chemistry, Hajdrihova 19, Ljubljana, Slovenia.
Slovenian NMR Center, National Institute of Chemistry, Hajdrihova 19, Ljubljana, Slovenia; University of Ljubljana, Faculty of Chemistry and Chemical Technology, Ljubljana, Slovenia; EN-FIST Centre of Excellence, Cesta OF 13, Ljubljana, Slovenia.
Curr Opin Struct Biol. 2023 Apr;79:102532. doi: 10.1016/j.sbi.2023.102532. Epub 2023 Feb 4.
RNA was shown to have a more substantial role in the regulation of diverse cellular processes than anticipated until recently. Answers to questions what is the structure of specific RNAs, how structure changes to accommodate different functional roles, and how RNA senses other biomolecules and changes its fold upon interaction create a complete representation of RNA involved in cellular processes. Nuclear magnetic resonance (NMR) spectroscopy encompasses a collection of methods and approaches that offer insight into several structural aspects of RNAs. We review the most recent advances in the field of viral, long non-coding, regulatory, and four-stranded RNAs, with an emphasis on the detection of dynamic sub-states and in view of chemical modifications that expand RNA's function.
直到最近，人们才发现RNA在多种细胞过程的调控中所起的作用比预期的更为重要。特定RNA的结构是什么、结构如何变化以适应不同的功能角色、RNA如何感知其他生物分子并在相互作用时改变其折叠方式，这些问题的答案构成了参与细胞过程的RNA的完整表征。核磁共振（NMR）光谱学包含一系列方法和途径，可深入了解RNA的几个结构方面。我们综述了病毒RNA、长链非编码RNA、调控RNA和四链RNA领域的最新进展，重点是动态亚状态的检测以及考虑到扩展RNA功能的化学修饰。
