The Key Laboratory of Myocardial Ischemia Organization, Chinese Ministry of Education, Harbin, Heilongjiang 150086, China; Department of Cardiology Organization, The Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150086, China.
The Key Laboratory of Myocardial Ischemia Organization, Chinese Ministry of Education, Harbin, Heilongjiang 150086, China; Department of Cardiology Organization, The Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150086, China.
Genomics. 2023 Mar;115(2):110573. doi: 10.1016/j.ygeno.2023.110573. Epub 2023 Feb 4.
Considerable studies have given convincing evidence of a forefront position for vascular aging in preventing cardiovascular disease. Various functions of Long non-coding RNAs (lncRNAs) are becoming increasingly distinct in aging-related diseases. This study aims at a better insight into the expression profile and mechanisms of lncRNAs in vascular senescence. High-throughput sequencing was used to detect the differential expression (DE) of lncRNAs and mRNAs in the aorta of 96 W and 8 W-old mice, while 1423 lncRNAs and 80 mRNAs were differentially expressed. By performing GO and KEGG enrichment analysis, we found that DE lncRNAs were mainly involved in purine metabolism and cGMP-PKG signaling pathways. In addition, a co-expression functional network of DE lncRNAs and DE mRNAs was constructed, and ENSMUST00000218874 could interact with 41 DE mRNAs, suggesting that it may play an essential role in vascular senescence. This study reveals DE lncRNAs in naturally aging vascular, which may provide new ideas and targets for aging-related cardiovascular diseases.
大量研究为血管衰老在预防心血管疾病中的前沿地位提供了令人信服的证据。长链非编码 RNA（lncRNA）的各种功能在与衰老相关的疾病中的作用越来越明显。本研究旨在更深入地了解 lncRNA 在血管衰老中的表达谱和机制。高通量测序用于检测 96 周龄和 8 周龄小鼠主动脉中 lncRNA 和 mRNA 的差异表达，发现有 1423 个 lncRNA 和 80 个 mRNA 存在差异表达。通过进行 GO 和 KEGG 富集分析，我们发现差异表达的 lncRNA 主要参与嘌呤代谢和 cGMP-PKG 信号通路。此外，构建了差异表达 lncRNA 和 mRNA 的共表达功能网络，发现 ENSMUST00000218874 可以与 41 个差异表达的 mRNA 相互作用，提示其可能在血管衰老中发挥重要作用。本研究揭示了自然衰老血管中的差异表达 lncRNA，为与衰老相关的心血管疾病提供了新的思路和靶点。
