椎间盘退变中与髓核相关的 lncRNA 和 mRNA 表达谱。

Nucleus pulposus related lncRNA and mRNA expression profiles in intervertebral disc degeneration.

机构信息

Shunde Hospital, Southern Medical University (The First People's Hospital of Shunde), Foshan, China.

Department of Orthopedics, The Affiliated Traditional Chinese Medicine Hospital of Southwest Medical University, Luzhou, China.

出版信息

Genomics. 2023 Mar;115(2):110570. doi: 10.1016/j.ygeno.2023.110570. Epub 2023 Feb 4.

DOI:10.1016/j.ygeno.2023.110570

PMID:36746221

Abstract

In the present study, we aimed to have a comprehensive understanding of nucleus pulposus related long noncoding RNA (lncRNA) and mRNA expression profiles in intervertebral disc degeneration (IDD). In total, 2418 mRNAs and 528 lncRNAs were found to be differentially expressed in the IDD group compared with the Control group. Combining microarray datasets and sequencing data, 5 overlapping DEMs and 7 overlapping DELs were identified. NF-κB signaling pathway, PI3K-Akt signaling pathway and Wnt/β-catenin signaling pathway were strongly linked with enriched GO terms and KEGG pathways. The ceRNA network suggested that lnc-TMEM44-AS1-hsa-miR-206-HDAC4 may be one crucial axis in IDD. PPI network analysis was constructed with 309 nodes and 129 edges. And the highest connectivity degrees were ALB, APOB and CCL2. This study suggested that specific lncRNAs and ceRNA axes may be crucial in the development of IDD. It provides a new perspective for delaying IDD process and enhancing intervertebral disc repair.

摘要

在本研究中，我们旨在全面了解椎间盘退变（IDD）中与髓核相关的长链非编码 RNA（lncRNA）和 mRNA 的表达谱。与对照组相比，IDD 组中共有 2418 个 mRNAs 和 528 个 lncRNAs 表达差异。结合微阵列数据集和测序数据，鉴定出 5 个重叠的 DEMs 和 7 个重叠的 DELs。NF-κB 信号通路、PI3K-Akt 信号通路和 Wnt/β-catenin 信号通路与富集的 GO 术语和 KEGG 通路密切相关。ceRNA 网络表明，lnc-TMEM44-AS1-hsa-miR-206-HDAC4 可能是 IDD 中的一个关键轴。构建了包含 309 个节点和 129 条边的 PPI 网络分析。并且具有最高连接度的是 ALB、APOB 和 CCL2。本研究表明，特定的 lncRNAs 和 ceRNA 轴可能在 IDD 的发展中起关键作用。它为延缓 IDD 进程和增强椎间盘修复提供了新的视角。