Wang Huilei, Li Xiaomei, Xia Bowei, Zhang Qingcui, He Junwei, Yang Li
College of Pharmacy, Jiangxi University of Chinese Medicine, Nanchang, 330004, China; Research Center of Natural Resources of Chinese Medicinal Materials and Ethnic Medicine, Jiangxi University of Chinese Medicine, Nanchang, 330004, China.
Research Center of Natural Resources of Chinese Medicinal Materials and Ethnic Medicine, Jiangxi University of Chinese Medicine, Nanchang, 330004, China.
J Ethnopharmacol. 2023 May 10;307:116245. doi: 10.1016/j.jep.2023.116245. Epub 2023 Feb 4.
Hosta plantaginea (Lam.) Aschers flower is an important Mongolian medicine beneficial in the treatment of chronic prostatitis (CP) in the absence of scientific evidence.
The aim of this study was to reveal the therapeutical effects and potential mechanisms of H. plantaginea flowers extract (HP) and its different polarity fractions (HPA∼D) on autoimmune CP (ACP) model rats.
Sprague-Dawley male rats were randomly assigned to 13 groups (n = 6/group). Except the sham group, all rats were injected with a mixture of prostate antigen and complete Freund's adjuvant on days 0, 7, and 21 to establish ACP model rats. Afterwards, ACP model rats were orally gavaged with HP or HPA∼D (1 and 4 g/kg of raw herbal material) or positive drug (Prostat, 200 mg/kg) daily from day 21 to day 50 for 30 days, while the sham and model groups were treated simultaneously with isopyknic of 0.3% sodium carboxymethyl cellulose. Histopathological analysis, biochemical parameters, and protein expression of prostate tissues were investigated.
In comparison with the model group, all fraction groups experienced improved CP effects, including restored body weight, reduced prostate gland edema and prostate index, decreased prostatic leukocytes, increased prostatic lecithin bodies, and alleviated histopathological damage to prostate tissue. Furthermore, all fraction groups markedly inhibited the phosphorylated protein of nuclear factor kappa-B p65 (NF-κB p65), NF-κB inhibitor alpha (IκBα), c-Jun N-terminal kinase (JNK), p38 mitogen-activated protein kinase (MAPK), extracellular signal-regulated kinase (Erk), just another kinase 1 (JAK1), signal transducer and activator of transcription 3 (STAT3), phosphoinositide 3-kinase (PI3K), and protein kinase B (Akt) than the model group.
All fractions of HP exerted significant anti-CP effects by inhibiting NF-κB, MAPKs, JAK-STAT and PI3K-Akt pathways in ACP model rats. These findings provide scientific evidence that H. plantaginea flowers can be used as a pivotal Mongolian medicine in clinical applications for the treatment of CP.
玉簪花是一种重要的蒙药，在缺乏科学证据的情况下，其对慢性前列腺炎（CP）具有治疗作用。
本研究旨在揭示玉簪花提取物（HP）及其不同极性组分（HPA∼D）对自身免疫性CP（ACP）模型大鼠的治疗效果及潜在机制。
将Sprague-Dawley雄性大鼠随机分为13组（每组n = 6）。除假手术组外，所有大鼠在第0、7和21天注射前列腺抗原与完全弗氏佐剂的混合物，以建立ACP模型大鼠。之后，从第21天至第50天，对ACP模型大鼠每日灌胃给予HP或HPA∼D（生药1和4 g/kg）或阳性药物（前列康，200 mg/kg），持续30天，而假手术组和模型组同时给予等体积的0.3%羧甲基纤维素钠。对前列腺组织进行组织病理学分析、生化参数检测及蛋白表达研究。
与模型组相比，所有组分组的CP症状均有改善，包括体重恢复、前列腺水肿减轻、前列腺指数降低、前列腺白细胞减少、前列腺卵磷脂小体增加以及前列腺组织病理损伤减轻。此外，所有组分组均比模型组显著抑制核因子κB p65（NF-κB p65）、NF-κB抑制因子α（IκBα）、c-Jun氨基末端激酶（JNK）、p38丝裂原活化蛋白激酶（MAPK）、细胞外信号调节激酶（Erk）、Janus激酶1（JAK1）、信号转导子和转录激活子3（STAT3）、磷脂酰肌醇3激酶（PI3K）和蛋白激酶B（Akt）的磷酸化蛋白。
HP的所有组分通过抑制ACP模型大鼠中的NF-κB、MAPKs、JAK-STAT和PI3K-Akt途径发挥显著的抗CP作用。这些发现为玉簪花可作为治疗CP的关键蒙药应用于临床提供了科学依据。
