Jiang Peng, Ji Xiang, Xia Jing, Xu Mengran, Hao Fang, Tong Haibin, Jiao Lili
Agriculture Gene Engineering Research Center of the Ministry of Education, Northeast Normal University, Changchun 130024, China.
Department of Chinese Osteo-traumatology, The Third Affiliated Hospital of Wenzhou Medical University, Wenzhou 325200, China.
Carbohydr Polym. 2023 Apr 15;306:120608. doi: 10.1016/j.carbpol.2023.120608. Epub 2023 Jan 20.
Two polysaccharides, BCP-1 and BCP-2, were obtained from Bupleurum chinense DC. by water extraction and ultrafiltration. BCP-1 (1.04 × 10 Da) and BCP-2 (2.14 × 10 Da) were composed of Mannose, Rhamnose, Glucose, Galactose, Arabinose, and Galacturonic acid in different proportions. They both contained oligogalacturonides in their main chain. Besides, the backbone of BCP-1 was composed of 4-β-Galp and 4,6-β-Glcp, and branched at C4 of 4,6-β-Glcp. While BCP-2 contained a backbone of 3,5-α-Araf residues with branches at C3. BCP-2 effectively extended the forced swimming time, improved the glycogen reserves and antioxidant system, decreased the levels of blood urea nitrogen, lactic acid, lactate dehydrogenase and creatinine kinase expression. It alleviated physical fatigue through regulating 5'-AMP-activated protein kinase (AMPK) and Nuclear Factor erythroid 2-Related Factor 2 (Nrf2) signalling pathway in skeletal muscles. This study demonstrated that BCP-2 exhibited more effective anti-fatigue activity than BCP-1 potentially associated with its primary and higher structures.
通过水提取和超滤从柴胡中获得了两种多糖，BCP-1和BCP-2。BCP-1（1.04×10 Da）和BCP-2（2.14×10 Da）由甘露糖、鼠李糖、葡萄糖、半乳糖、阿拉伯糖和半乳糖醛酸按不同比例组成。它们的主链中均含有低聚半乳糖醛酸。此外，BCP-1的主链由4-β-半乳糖和4,6-β-葡萄糖组成，并在4,6-β-葡萄糖的C4位分支。而BCP-2含有由3,5-α-阿拉伯糖残基组成的主链，在C3位分支。BCP-2有效延长了强迫游泳时间，改善了糖原储备和抗氧化系统，降低了血尿素氮、乳酸、乳酸脱氢酶和肌酸激酶的表达水平。它通过调节骨骼肌中的5'-AMP激活蛋白激酶（AMPK）和核因子红细胞2相关因子2（Nrf2）信号通路来减轻身体疲劳。本研究表明，BCP-2表现出比BCP-1更有效的抗疲劳活性，这可能与其一级和高级结构有关。
