Corporación Colombiana de Investigación Agropecuaria (AGROSAVIA). Centro de Investigación Nataima, Km 9, Espinal-Ibagué, Tolima, Colombia.
Universidad del Tolima, Cl. 42 #1b-1, Ibagué, Tolima, Colombia.
Arch Microbiol. 2023 Feb 6;205(3):83. doi: 10.1007/s00203-023-03405-2.
The aim of this study was to evaluate and select entomopathogenic fungi that produces insecticidal compounds for the control of adults of Anastrepha obliqua Macquart (Diptera: tephritidae) that are the main pest of mango (Mangifera indica L. Bark) in Colombia. Nine entomopathogenic fungi isolates were evaluated, five belonging to the genus Metarhizium and four belonging to the genus Beauveria. One strain of the species Metarhizium robertsii with insecticidal activity was selected. By column fractionation, an active fraction was obtained, which caused mortalities higher than 90% after 48 h of exposure. Through HPLC it was determined that the active fraction is composed of more than 22 metabolites. Identification of the metabolites by UHPLC MS/MS revealed the presence of destruxin in E, D, A and B groups (destruxin E-diol, destruxin D, destruxin D1, destruxin D2, destruxin A2, destruxin A, destruxin A3, dihydrodestruxin A, desmB, destruxin B2, destruxin B and destruxin B1). The evaluation of the insecticidal capacity of the organic fractions obtained by HPLC indicated that the extract obtained from the isolate M. robertsii had a compound with high activity on adults of A. obliqua (destruxin A) causing massive mortality of up to 100%, after 48 h of the treatment administration. Furthermore, two other compounds with medium activity were found (destruxin A2 and destruxin B), showing mortalities between 60.0 and 81.3%, respectively. The extract of the isolate MT008 of M. robertsii showed higher insecticidal activity and a potential source for the control of A. obliqua.
本研究旨在评估和筛选产生杀虫化合物的昆虫病原真菌，以防治哥伦比亚芒果（Mangifera indica L. Bark）主要害虫——番石榴实蝇（Anastrepha obliqua Macquart）成虫。评估了 9 种昆虫病原真菌分离株，其中 5 种属于绿僵菌属，4 种属于白僵菌属。筛选出一种具有杀虫活性的绿僵菌属罗伯茨绿僵菌（Metarhizium robertsii）菌株。通过柱层析分离，得到一个活性馏分，该馏分在暴露 48 小时后死亡率超过 90%。通过 HPLC 确定，该活性馏分由 22 种以上代谢物组成。通过 UHPLC MS/MS 对代谢物进行鉴定，发现 E、D、A 和 B 组中存在毁灭霉素（destruxin），包括 destruxin E-diol、destruxin D、destruxin D1、destruxin D2、destruxin A2、destruxin A、destruxin A3、dihydrodestruxin A、desmB、destruxin B2、destruxin B 和 destruxin B1。通过 HPLC 获得的有机馏分杀虫能力评估表明，从 M. robertsii 分离株获得的提取物含有一种对 A. obliqua 成虫活性很高的化合物（destruxin A），在处理后 48 小时内导致成虫大量死亡，死亡率高达 100%。此外，还发现了两种中等活性的化合物（destruxin A2 和 destruxin B），死亡率分别为 60.0%和 81.3%。M. robertsii 的 MT008 分离株的提取物显示出更高的杀虫活性，是防治 A. obliqua 的潜在来源。
