Department of Biochemistry and Biotechnology, University of Thessaly, Larissa 41221, Greece.
Pest Manag Sci. 2010 Apr;66(4):447-53. doi: 10.1002/ps.1921.
Among target pests of the insecticide spinosad is the olive fruit fly, Bactrocera oleae (Rossi) (Diptera: Tephritidae). In Cyprus, spinosad has been sporadically used since its registration in 2002, whereas in Greece its use has been very limited since its registration in 2004, particularly in biological olive cultivars in Crete. By contrast, in California it has been the only insecticide used against the olive fruit fly since its registration in 2004. This study aimed at examining the resistance status of the olive fruit fly to spinosad.
Populations from California, Greece and Cyprus, plus a laboratory population, were tested. Bioassays were performed by oral or topical application of different concentrations of the insecticide. Cypriot populations demonstrated no resistance as compared with that of the laboratory population. Among the Greek populations, only one from Crete demonstrated a fourfold increase in resistance, whereas five populations from California demonstrated a 9-13-fold increase.
The observed resistance increase was associated with spinosad applications in the respective areas. These values are relatively low and do not yet pose a serious control problem in the field. However, the observed variation documents that spinosad tolerance has increased in areas where the insecticide has been more extensively used.
杀虫剂多杀菌素的靶标害虫之一是橄榄果蝇，Bactrocera oleae（Rossi）（双翅目：瘿蚊科）。在塞浦路斯，自 2002 年注册以来，多杀菌素已零星使用，而在希腊，自 2004 年注册以来，其使用非常有限，特别是在克里特岛的生物橄榄品种中。相比之下，自 2004 年注册以来，它一直是加利福尼亚州防治橄榄果蝇的唯一杀虫剂。本研究旨在研究橄榄果蝇对多杀菌素的抗药性状况。
测试了来自加利福尼亚州、希腊和塞浦路斯的种群，以及一个实验室种群。通过口服或局部应用不同浓度的杀虫剂进行生物测定。与实验室种群相比，塞浦路斯种群没有表现出抗药性。在希腊种群中，只有一个来自克里特岛的种群显示出四倍的抗药性增加，而加利福尼亚州的五个种群显示出 9-13 倍的抗药性增加。
观察到的抗药性增加与各自地区的多杀菌素应用有关。这些值相对较低，在田间尚未构成严重的控制问题。然而，观察到的变异表明，在杀虫剂使用更广泛的地区，多杀菌素的耐受性有所增加。
