番茄 APETALA2 家族成员 SlTOE1 通过抑制 TM3 的 SISTER 来调控花序分枝。
Tomato APETALA2 family member SlTOE1 regulates inflorescence branching by repressing SISTER OF TM3.
机构信息
State Key Laboratory of Vegetable Biobreeding, Sino-Dutch Joint Laboratory of Horticultural Genomics, China Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Key Laboratory of Quality and Safety Control for Subtropical Fruit and Vegetable, Ministry of Agriculture and Rural Affairs, College of Horticulture Science, Zhejiang A&F University, Hangzhou, Zhejiang 311300, China.
出版信息
Plant Physiol. 2023 May 2;192(1):293-306. doi: 10.1093/plphys/kiad075.
Inflorescence architecture directly impacts yield potential in most crops. As a model of sympodial plants, tomato (Solanum lycopersicum) inflorescence exhibits highly structural plasticity. However, the genetic regulatory network of inflorescence architecture in tomato remains unclear. Here, we investigated a modulator of inflorescence branching in tomato, TARGET OF EAT1 (SlTOE1), an APETALA2 (AP2) family member found to be predominantly expressed in the floral meristem (FM) of tomato. sltoe1 knockout mutants displayed highly branched inflorescences and defective floral organs. Transcriptome analysis revealed that SISTER OF TM3 (STM3) and certain floral development-related genes were upregulated in the flower meristem of sltoe1. SlTOE1 could directly bind the promoters of STM3 and Tomato MADS-box gene 3 (TM3) to repress their transcription. Simultaneous mutation of STM3 and TM3 partially restored the inflorescence branching of the sltoe1cr mutants, suggesting that SlTOE1 regulates inflorescence development, at least in part through an SlTOE1STM3/TM3 module. Genetic analysis showed that SlTOE1 and ENHANCER OF JOINTLESS 2 (EJ2) additively regulate tomato inflorescence branching; their double mutants showed more extensive inflorescence branching. Our findings uncover a pathway controlling tomato inflorescence branching and offer deeper insight into the functions of AP2 subfamily members.
花序结构直接影响大多数作物的产量潜力。作为合轴植物的模式植物，番茄（Solanum lycopersicum）的花序表现出高度的结构可塑性。然而，番茄花序结构的遗传调控网络仍不清楚。在这里，我们研究了番茄花序分枝的调节剂，即 TARGET OF EAT1（SlTOE1），它是 APETALA2（AP2）家族的成员，在番茄的花分生组织（FM）中表达为主。sltoe1 敲除突变体表现出高度分枝的花序和有缺陷的花器官。转录组分析显示，SISTER OF TM3（STM3）和某些与花发育相关的基因在 sltoe1 的花分生组织中上调。SlTOE1 可以直接结合 STM3 和番茄 MADS-box 基因 3（TM3）的启动子，抑制它们的转录。STM3 和 TM3 的同时突变部分恢复了 sltoe1cr 突变体的花序分枝，表明 SlTOE1 通过 SlTOE1STM3/TM3 模块调节花序发育，至少部分如此。遗传分析表明，SlTOE1 和 ENHANCER OF JOINTLESS 2（EJ2）在番茄花序分枝中具有累加作用；它们的双突变体表现出更广泛的花序分枝。我们的研究结果揭示了一个控制番茄花序分枝的途径，并为 AP2 亚家族成员的功能提供了更深入的了解。
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