College of Horticulture and Gardening, Yangtze University, Jingzhou 434025, China.
Int J Mol Sci. 2024 Jun 29;25(13):7193. doi: 10.3390/ijms25137193.
In the rosid species , the AP2-type AP2 transcription factor (TF) is required for specifying the sepals and petals identities and confers a major A-function to antagonize the C-function in the outer floral whorls. In the asterid species , the AP2-type ROB TFs are required for perianth and pistil development, as well as repressing the B-function together with TOE-type TF BEN. In Long-homostyle (LH) , VIGS-silencing showed that is mainly involved in controlling filament and style length, but is mainly involved in regulating filament length and pollen grain development. Both (AP2-type) and (TOE-type) are redundantly involved in style and/or filament length determination instead of perianth development. However, neither nor could directly repress the B and/or C class genes in common buckwheat. Moreover, the silenced flower showed tepal numbers, and filament length decreased obviously. Interestingly, yeast one-hybrid (Y1H) and dual-luciferase reporter (DR) further suggested that directly up-regulates to be involved in filament length determination in LH common buckwheat. Moreover, the knockdown of expression could result in expression down-regulation of the directly target in the -silenced LH plants. Our findings uncover a stamen development pathway in common buckwheat and offer deeper insight into the functional evolution of AP2 orthologs in the early-diverging core eudicots.
在蔷薇目中，AP2 型 AP2 转录因子（TF）对于指定萼片和花瓣的身份是必需的，并赋予主要的 A 功能来拮抗外轮花器官中的 C 功能。在石竹目中，AP2 型 ROB TF 对于花被片和雌蕊的发育是必需的，同时与 TOE 型 TF BEN 一起抑制 B 功能。在长花柱（LH）中，VIGS 沉默表明 主要参与控制花丝和花柱长度，但 主要参与调节花丝长度和花粉粒发育。 （AP2 型）和 （TOE 型）都冗余地参与花柱和/或花丝长度的决定，而不是花被片的发育。然而，在苦荞中，既 也不能直接抑制 B 和/或 C 类基因。此外，沉默的花显示出花萼的数量减少，花丝长度明显缩短。有趣的是，酵母单杂交（Y1H）和双荧光素酶报告（DR）进一步表明 直接上调 参与 LH 苦荞花丝长度的决定。此外， 表达的敲低会导致 -沉默的 LH 植物中直接靶标 的表达下调。我们的发现揭示了苦荞中雄蕊发育的途径，并深入了解了早期分化的核心真双子叶植物中 AP2 同源物的功能进化。
