Zhang Liyuan, Voss Joachim G, Soulakova Julia N, St Pierre Schneider Barbara
College of Nursing and Health Innovation, University of Texas at Arlington, Arlington, Texas, USA.
Frances Payne Bolton School of Nursing, Case Western Reserve University, Cleveland, Ohio, USA.
Muscle Nerve. 2023 Apr;67(4):320-329. doi: 10.1002/mus.27795. Epub 2023 Feb 23.
INTRODUCTION/AIMS: Because wounded warfighters or trauma victims may receive en route care to the closest medical facility via airplane transport, we investigated the effects of extended mild hypobaric hypoxia (HB), the environmental milieu of most airplanes, on inflammation and regeneration after muscle trauma or monotrauma (MT) and muscle trauma-hemorrhagic shock or polytrauma (PT).
Male C57BL/6N mice were assigned to one of six groups pertaining to injury (control/uninjured, MT, and PT) and atmospheric pressure exposure (HB and normobaric normoxia, NB). Body mass, blood and muscle leukocyte number by flow cytometry, immunohistochemistry, or both, and the muscle relative mRNA level of selected genes involved in inflammation and muscle regeneration were examined at ~1.7, 4, 8, and 14 days post trauma (dpt). At 14 dpt, the proportion of smaller- and larger-sized myofibers at the regenerating site of MT mice was determined.
Greater body mass loss, an increased number of blood and muscle leukocytes, and differential muscle relative mRNA levels were observed in MT and PT groups compared to controls. The MT+HB or PT+HB mice demonstrated more body mass loss and altered relative mRNA level than the corresponding NB mice. Additionally, a subgroup of MT+HB mice demonstrated a greater proportion of smaller myofibers (250 to 500 μm ) than MT+NB mice at 14 dpt.
HB exposure after muscle trauma alone may prolong regeneration. Following HB exposure, therapies that promote oxygenation may be needed during this muscle recovery.
引言/目的：由于受伤的战士或创伤受害者可能通过飞机转运接受前往距离最近医疗机构的途中护理，我们研究了大多数飞机的环境条件——长时间轻度低压缺氧（HB）对肌肉创伤或单一创伤（MT）以及肌肉创伤-失血性休克或多发伤（PT）后炎症和再生的影响。
将雄性C57BL/6N小鼠分为与损伤（对照/未受伤、MT和PT）及气压暴露（HB和常压常氧，NB）相关的六组之一。在创伤后约1.7、4、8和14天（dpt），通过流式细胞术、免疫组织化学或两者检测体重、血液和肌肉白细胞数量，以及参与炎症和肌肉再生的选定基因的肌肉相对mRNA水平。在14 dpt时，测定MT小鼠再生部位较小和较大尺寸肌纤维的比例。
与对照组相比，MT组和PT组观察到更大的体重减轻、血液和肌肉白细胞数量增加以及不同的肌肉相对mRNA水平。与相应的NB小鼠相比，MT+HB或PT+HB小鼠表现出更多的体重减轻和相对mRNA水平改变。此外，在14 dpt时，MT+HB小鼠的一个亚组显示出比MT+NB小鼠更大比例的较小肌纤维（250至500μm）。
仅肌肉创伤后的HB暴露可能会延长再生时间。在HB暴露后，在肌肉恢复期间可能需要促进氧合的治疗方法。
