Trauma Department, Hannover Medical School, Hannover, Germany.
Department of Trauma, Hand and Reconstructive Surgery, University Hospital Frankfurt, Goethe University, Frankfurt, Germany.
Sci Rep. 2019 Apr 5;9(1):5706. doi: 10.1038/s41598-019-42085-1.
This study aims to elaborate the relevance of trauma severity and traumatic injury pattern in different multiple and/or polytrauma models by comparing five singular trauma to two different polytrauma (PT) models with high and one multiple trauma (MT) model with low injury-severity score (ISS). The aim is to provide a baseline for reducing animal harm according to 3Rs by providing less injury as possible in polytrauma modeling. Mice were randomly assigned to 10 groups: controls (Ctrl; n = 15), Sham (n = 15); monotrauma groups: hemorrhagic shock (HS; n = 15), thoracic trauma (TxT; n = 18), osteotomy with external fixation (Fx; n = 16), bilateral soft tissue trauma (bSTT; n = 16) or laparotomy (Lap; n = 16); two PT groups: PT I (TxT + HS + Fx; ISS = 18; n = 18), PT II (TxT + HS + Fx + Lap; ISS = 22; n = 18), and a MT group (TxT + HS + bSTT + Lap, ISS = 13; n = 18). Activity and mortality were assessed. Blood gas analyses and organ damage markers were determined after 6 h. Significant mortality occurred in TxT, PT and MT (11.7%). Activity decreased significantly in TxT, HS, both polytrauma and MT vs. Ctrl/Sham. PT-groups and MT had significantly decreased activity vs. bsTT, Lap or Fx. MT had significantly lower pCO vs. Ctrl/Sham, Lap or bsTT. Transaminases increased significantly in PT-groups and MT vs. Ctrl, Sham or monotrauma. Traumatic injury pattern is of comparable relevance as injury severity for experimental multiple or (poly)trauma modeling.
本研究旨在通过比较五种单一创伤与两种不同的高创伤严重程度（ISS）的多发伤（PT）模型和一种低损伤严重程度评分（ISS）的多发伤（MT）模型，阐述创伤严重程度和创伤损伤模式在不同多发伤和/或多发伤模型中的相关性。目的是根据 3R 原则，通过尽可能减少多发伤建模中的损伤，为减少动物伤害提供基线。将小鼠随机分为 10 组：对照组（Ctrl；n = 15）、假手术组（Sham；n = 15）；单一创伤组：失血性休克（HS；n = 15）、胸部创伤（TxT；n = 18）、外固定骨折（Fx；n = 16）、双侧软组织创伤（bSTT；n = 16）或剖腹术（Lap；n = 16）；两个 PT 组：PT I（TxT + HS + Fx；ISS = 18；n = 18），PT II（TxT + HS + Fx + Lap；ISS = 22；n = 18）和一个 MT 组（TxT + HS + bSTT + Lap，ISS = 13；n = 18）。评估活动度和死亡率。6 小时后进行血气分析和器官损伤标志物测定。TxT、PT 和 MT 组死亡率显著升高（11.7%）。与 Ctrl/Sham 相比，TxT、HS、PT 和 MT 组的活动度显著降低。PT 组和 MT 组的活动度与 bsTT、Lap 或 Fx 相比显著降低。与 Ctrl/Sham、Lap 或 bsTT 相比，MT 组的 pCO 显著降低。与 Ctrl、Sham 或单一创伤相比，PT 组和 MT 组的转氨酶显著升高。创伤损伤模式与实验性多发伤或（多发）创伤模型的损伤严重程度具有同等的相关性。
