Lindahl B, Alm P, Killander D, Långström E, Tropé C
Department of Obstetrics and Gynecology, University Hospital, Lund, Sweden.
Anticancer Res. 1987 Jul-Aug;7(4B):781-9.
The DNA content in individual cells from 112 histopathologically normal endometria and from 222 malignant endometrial tumors was measured using flow cytometry. In the normal endometrial cases only single DNA peaks were found, all in the diploid region, and the range of DNA index values was used for defining the limits for diploid tumor cases. In most cases with aneuploid cell populations, an additional peak was found in the normal diploid region. However, combined cytological and histopathological analysis showed that a majority of these diploid cells were to be considered as tumor cells. Aneuploid cell populations were found in 43% of malignant endometria; in the remaining endometrial carcinomas, the flow cytometrical findings showed no differences compared to those of benign tissue. Flow cytometry in this respect did not prove useful as a diagnostic screening method. Ploidy aberrations were correlated to histopathology. Aneuploidy was more common (62%) in poorly differentiated tumors than in highly/moderately differentiated tumors (29%). Two or more aneuploid cell populations were found in 6% of the cases. No difference in aneuploidy was found between FIGO stage I and II (36% and 34%), but aneuploidy was more frequent in stage IV (86%). In normal endometria the fraction of cells with DNA content corresponding to S-phase (S-fraction) was 9.7% on average in the proliferative phase and 6.2% in the secretory phase. In well and moderately differentiated diploid tumors the S-fraction was about the same (8.8% and 9.2%), but in poorly differentiated tumors it was significantly higher (12-16.5%).
采用流式细胞术测量了112例组织病理学正常的子宫内膜以及222例恶性子宫内膜肿瘤中单个细胞的DNA含量。在正常子宫内膜病例中，仅发现单个DNA峰，均位于二倍体区域，DNA指数值范围用于定义二倍体肿瘤病例的界限。在大多数非整倍体细胞群体的病例中，在正常二倍体区域发现了一个额外的峰。然而，细胞学和组织病理学联合分析表明，这些二倍体细胞中的大多数应被视为肿瘤细胞。43%的恶性子宫内膜中发现了非整倍体细胞群体；在其余的子宫内膜癌中，流式细胞术的结果与良性组织相比无差异。在这方面，流式细胞术作为一种诊断筛查方法并无用处。倍性异常与组织病理学相关。未分化肿瘤中出现非整倍体的情况更为常见（62%），高于高/中分化肿瘤（29%）。6%的病例中发现了两个或更多的非整倍体细胞群体。国际妇产科联盟（FIGO）I期和II期之间的非整倍体情况无差异（分别为36%和34%），但IV期更为常见（86%）。在正常子宫内膜中，增殖期DNA含量对应于S期（S期分数）的细胞比例平均为9.7%，分泌期为6.2%。在高分化和中分化二倍体肿瘤中，S期分数大致相同（分别为8.8%和9.2%），但在未分化肿瘤中显著更高（12 - 16.5%）。
