Aragaw Girmay, Chala Alemayehu, Terefe Habtamu
Department of Plant Sciences, Debre Tabor University, P.O. Box 272, Debre Tabor, Ethiopia.
College of Agriculture, Hawassa University, P.O. Box 5, Hawassa, Ethiopia.
Heliyon. 2023 Jan 16;9(1):e13057. doi: 10.1016/j.heliyon.2023.e13057. eCollection 2023 Jan.
is the most destructive pathogen causing sorghum anthracnose worldwide. The fungus is known to have highly variable pathotypes. A characteristic study of pathogen is important to document the change occurring in population as variability in morphology indicates the existence of different pathotypes. Controlled condition experiment was conducted to examine cultural and morphological characteristics of isolates infecting sorghum in eastern Ethiopia. Sorghum leaves showing symptoms of anthracnose were collected from five districts through survey. To study the characteristics of , single-spore isolates representing isolate collection districts were selected from the stock cultures and cultivated on potato dextrose agar. Culture growth, colony color, elevations, texture and margin, conidial diameter and shape were used to characterize isolates. The isolates were varied significantly in many aspects. Colony colors were differed from light-gray to gray, purple-gray to cottony-gray, white to salmon-whit, plum-pink to beige and rosy brown on upper side of the petri dishes. The mean culture growth of isolates showed highly significant (P < 0.01) variations among each other and ranged from 15 to 44 mm eight-days after incubation. Most of the isolates were produced hyaline, smooth walled, falcate conidia but without septa. Conidial diameter of isolates showed variations with width and length ranged from 2.97 to 6.01 μm and 10.01-27.75 μm, respectively. Most isolates had smooth colony margin and few had undulated margin. This finding revealed that substantial variations were observed among isolates and the existence of variable characteristic showed the presence of several sub-species of the pathogen infecting sorghum in different agro-ecologies of eastern Ethiopia.
是全球范围内引起高粱炭疽病最具破坏性的病原菌。已知该真菌具有高度可变的致病型。对病原菌进行特征研究对于记录种群中发生的变化很重要，因为形态学上的变异性表明存在不同的致病型。进行了控制条件实验，以研究感染埃塞俄比亚东部高粱的分离株的培养和形态特征。通过调查从五个地区收集了表现出炭疽病症状的高粱叶片。为了研究的特征，从保藏培养物中选择代表分离株收集地区的单孢分离株，并在马铃薯葡萄糖琼脂上培养。培养生长、菌落颜色、凸起、质地和边缘、分生孢子直径和形状用于表征分离株。分离株在许多方面存在显著差异。在培养皿上侧，菌落颜色从浅灰色到灰色、紫灰色到棉灰色、白色到鲑鱼白色、李子粉色到米色以及玫瑰棕色不等。分离株的平均培养生长在彼此之间显示出极显著（P < 0.01）的差异，培养八天后范围为15至44毫米。大多数分离株产生透明、壁光滑、镰刀形的分生孢子，但无隔膜。分离株的分生孢子直径在宽度和长度上存在差异，分别为2.97至6.01微米和10.01 - 27.75微米。大多数分离株的菌落边缘光滑，少数有波浪状边缘。这一发现表明，在分离株中观察到了显著差异，可变特征的存在表明在埃塞俄比亚东部不同农业生态环境中感染高粱的病原菌存在几个亚种。
