新种和新种，从青藏高原的仁龙巴冰川分离得到。

sp. nov. and sp. nov., isolated from Renlongba glacier on the Tibetan Plateau.

作者信息

Yang Lei-Lei, Pang Yu, Liu Hong-Can, Xin Yu-Hua, Liu Qing

机构信息

China General Microbiological Culture Collection Center, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.

University of Science and Technology Beijing, Beijing 100083, PR China.

出版信息

Int J Syst Evol Microbiol. 2022 Dec;72(12). doi: 10.1099/ijsem.0.005669.

DOI:10.1099/ijsem.0.005669

PMID:36748418

Abstract

Strains RB4R14 and RT5R15, two Gram-stain-negative, aerobic, rod-shaped, non-motile bacteria, were isolated from ice and cryoconite of Renlongba glacier, respectively, on the Tibetan Plateau, PR China. The results of phylogenetic analysis based on 16S rRNA genes indicated that strains RB4R14 and RT5R15 belonged to the genus with the highest similarities to WPCB133 (98.78 %) and BJC16-A31 (97.64 %), respectively. The genomic DNA G+C contents of strains RB4R14 and RT5R15 were 42.8 and 43.1 mol%, respectively. The digital DNA-DNA hybridization values between strains RB4R14, RT5R15 and their close relatives were below 31.9 and 17.4 %, respectively. The average nucleotide identity values between the two novel strains and their close relatives were 79.5-82.0 and 77.9-79.3 % respectively, indicating the novelty of the two isolates at a species level. The two novel strains contained MK-7 as the major menaquinone, and summed feature 3 (comprising C 7 and/or C 6), iso-C and iso-C-3OH as the major fatty acids. The major polar lipid of the two novel strains were phosphatidylethanolamine. Based on phenotypic, chemotaxonomic and phylogenetic results, we propose two novel species, sp. nov. (RB4R14=CGMCC 1.11911=NBRC 114020) and sp. nov. (RT5R15=CGMCC 1.23117=NBRC 113930).

摘要

菌株RB4R14和RT5R15是两株革兰氏染色阴性、需氧、杆状、无运动性的细菌，分别从中国青藏高原仁龙巴冰川的冰和冰尘中分离得到。基于16S rRNA基因的系统发育分析结果表明，菌株RB4R14和RT5R15分别属于与WPCB133（相似度98.78%）和BJC16 - A31（相似度97.64%）最相似的属。菌株RB4R14和RT5R15的基因组DNA G + C含量分别为42.8和43.1 mol%。菌株RB4R14、RT5R15与其近缘菌株之间的数字DNA - DNA杂交值分别低于31.9%和17.4%。这两株新菌株与其近缘菌株之间的平均核苷酸同一性值分别为79.5 - 82.0%和77.9 - 79.3%，表明这两个分离株在种水平上具有新颖性。这两株新菌株以MK - 7作为主要甲基萘醌，以总和特征3（包含C17:1ω6c和/或C16:1ω7c）、异C15:0和iso-C15:0 3-OH作为主要脂肪酸。这两株新菌株的主要极性脂为磷脂酰乙醇胺。基于表型、化学分类和系统发育结果，我们提出两个新物种，即RB4R14新种（RB4R14 = CGMCC 1.11911 = NBRC 114020）和RT5R15新种（RT5R15 = CGMCC 1.23117 = NBRC 113930）。