sp. nov.，一种从青藏高原的仁龙巴冰川中分离出来的耐冷细菌。
sp. nov., a psychrotolerant bacterium isolated from Renlongba glacier on the Tibetan Plateau.
机构信息
China General Microbiological Culture Collection Center, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
出版信息
Int J Syst Evol Microbiol. 2020 Feb;70(2):1031-1036. doi: 10.1099/ijsem.0.003868.
One aerobic, Gram-stain-negative, rod-shaped bacterium, designated strain RB1R5, was isolated from Renlongba glacier in Tibet Autonomous Region, China. Growth was observed at 4-25 °C and pH 7.0-8.0. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain RB1R5 belongs to the genus , and its closest relatives are IMCC26026 and CCM 8827 with 97.75 and 97.42% gene sequence similarities, respectively. The average nucleotide identity and DNA-DNA hybridization values between strain RB1R5 and its closest relative IMCC26026 were 78.02 and 21.8 %, respectively. The major cellular fatty acids were anteiso-C and summed feature 3 (comprising Cω7/Cω6). The sole menaquinone was MK-6. The major phospholipid was phosphatidylethanolamine. On the basis of phenotypic, phylogenetic and chemotaxonomic characterizations, strain RB1R5 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is RB1R5 (=CGMCC 1.23024=NBRC 113060).
一株好氧、革兰氏阴性、杆状细菌，被命名为 RB1R5 菌株，从中国西藏自治区的热隆巴冰川中分离得到。该菌在 4-25℃和 pH7.0-8.0 的条件下生长。基于 16S rRNA 基因序列的系统发育分析表明，菌株 RB1R5 属于 属，与 IMCC26026 和 CCM 8827 的亲缘关系最近，相似性分别为 97.75%和 97.42%。菌株 RB1R5 与其最接近的亲缘关系 IMCC26026 的平均核苷酸同一性和 DNA-DNA 杂交值分别为 78.02%和 21.8%。主要细胞脂肪酸为 anteiso-C 和总和特征 3（包含 Cω7/Cω6）。唯一的menaquinone 是 MK-6。主要磷脂是磷脂酰乙醇胺。基于表型、系统发育和化学分类特征，菌株 RB1R5 代表了 属的一个新种，提议将其命名为 sp. nov.。模式菌株为 RB1R5（=CGMCC 1.23024=NBRC 113060）。
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