Department of Biochemistry, Qing Hai University Medical College, Xi Ning, China.
Baoding First Central Hospital, Bao Ding, China.
High Alt Med Biol. 2023 Mar;24(1):59-67. doi: 10.1089/ham.2022.0095. Epub 2023 Feb 7.
Liu, Fang, Caiyan Hu, Jin Ding, Chengbing Fu, Shuqiong Wang, and Tiantian Li. GATA-1 promotes erythroid differentiation through the upregulation of and expression in CD34 cells in high-altitude polycythemia. . 24:59-67, 2023. The clinical manifestations of high-altitude polycythemia (HAPC) include excessive accumulation of erythrocytes, and its pathogenesis is not yet clear. Peripheral blood was collected from 10 HAPC patients (HAPC group) and normal individuals (control group) each. CD34 cells were sorted using immunomagnetic beads and differentiated into erythroid cells for 7, 11, and 15 days. Changes in GATA-binding protein 1 (GATA-1), , and expression and their possible regulatory relationships were investigated. Under hypoxia, GATA-1 expression on day 15 was about 2.4 times that on day 7 in the control group and about 1.3 times that on day 7 in the HAPC group, which was significantly lower compared with the control group. and expressions in the HAPC group were 2.6 and 1.8 times that in the control group, respectively, and were significantly increased. When GATA-1 was inhibited, and expressions were significantly decreased by 0.43 and 0.39 times, respectively, compared with those in the control group. Hypoxia stimulated the upregulation of GATA-1 level and accelerated the change of expression, which promoted and expressions and shortened the time taken by cells to enter end-stage differentiation, so as to enhance erythroid differentiation, which may be a pathogenetic mechanism underlying HAPC polycytosis.
刘芳，胡彩艳，丁进，傅成兵，王述琼，李天天。GATA-1 通过上调 CD34 细胞中 和 的表达促进红细胞分化在高原红细胞增多症中。. 24:59-67, 2023. 高原红细胞增多症 (HAPC) 的临床表现包括红细胞过度堆积，其发病机制尚不清楚。 从 10 例 HAPC 患者（HAPC 组）和 10 例正常个体（对照组）中采集外周血。使用免疫磁珠分选 CD34 细胞，并将其分化为红细胞，分别培养 7、11 和 15 天。研究了 GATA 结合蛋白 1 (GATA-1)、 和 表达的变化及其可能的调节关系。 在低氧条件下，对照组第 15 天 GATA-1 的表达约为第 7 天的 2.4 倍，HAPC 组第 7 天的 1.3 倍，与对照组相比明显降低。 HAPC 组 和 的表达分别是对照组的 2.6 倍和 1.8 倍，均明显升高。当抑制 GATA-1 时，与对照组相比， 和 的表达分别下降了 0.43 和 0.39 倍。 低氧刺激 GATA-1 水平上调并加速表达变化，促进 和 表达，缩短细胞进入终末分化所需时间，从而增强红细胞分化，这可能是 HAPC 红细胞增多症的发病机制之一。
