GATA-1 Promotes Erythroid Differentiation Through the Upregulation of and Expressions in CD34 Cells in High-Altitude Polycythemia.

Liu, Fang, Caiyan Hu, Jin Ding, Chengbing Fu, Shuqiong Wang, and Tiantian Li. GATA-1 promotes erythroid differentiation through the upregulation of and expression in CD34 cells in high-altitude polycythemia. . 24:59-67, 2023. The clinical manifestations of high-altitude polycythemia (HAPC) include excessive accumulation of erythrocytes, and its pathogenesis is not yet clear. Peripheral blood was collected from 10 HAPC patients (HAPC group) and normal individuals (control group) each. CD34 cells were sorted using immunomagnetic beads and differentiated into erythroid cells for 7, 11, and 15 days. Changes in GATA-binding protein 1 (GATA-1), , and expression and their possible regulatory relationships were investigated. Under hypoxia, GATA-1 expression on day 15 was about 2.4 times that on day 7 in the control group and about 1.3 times that on day 7 in the HAPC group, which was significantly lower compared with the control group. and expressions in the HAPC group were 2.6 and 1.8 times that in the control group, respectively, and were significantly increased. When GATA-1 was inhibited, and expressions were significantly decreased by 0.43 and 0.39 times, respectively, compared with those in the control group. Hypoxia stimulated the upregulation of GATA-1 level and accelerated the change of expression, which promoted and expressions and shortened the time taken by cells to enter end-stage differentiation, so as to enhance erythroid differentiation, which may be a pathogenetic mechanism underlying HAPC polycytosis.