人表皮中的糖缀合物生物合成

Glycoconjugate biosynthesis in human epidermis.

作者信息

Roberts G P

机构信息

Department of Medicine, University of Wales College of Medicine, Heath Park, Cardiff, U.K.

出版信息

Arch Dermatol Res. 1987;279(4):251-6. doi: 10.1007/BF00417324.

DOI:10.1007/BF00417324

PMID:3674958

Abstract

Epidermal glycoconjugates synthesized by skin slices in media containing radiolabelled sugars were analysed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis. A large proportion of the epidermal glycoconjugates were soluble in 10% (w/v) trichloroacetic acid. In addition to glycolipids and glycosaminoglycans, the acid-soluble fraction contained glycoproteins, a group of which (mol. wt. 70,000-160,000) were highly glycosylated and particularly rich in fucose. Analysis of the glycosaminoglycans by cellulose acetate electrophoresis combined with specific enzymic and nitrous acid degradation studies revealed that hyaluronic acid (73%) and heparan sulphate (18%) were the main components.

摘要

通过在含有放射性标记糖的培养基中培养皮肤切片合成的表皮糖缀合物，采用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳进行分析。大部分表皮糖缀合物可溶于10%（w/v）的三氯乙酸。除糖脂和糖胺聚糖外，酸溶性部分还含有糖蛋白，其中一组（分子量70,000 - 160,000）糖基化程度很高，且岩藻糖含量特别丰富。通过醋酸纤维素电泳结合特定的酶解和亚硝酸降解研究对糖胺聚糖进行分析，结果表明透明质酸（73%）和硫酸乙酰肝素（18%）是主要成分。