. Faculdade de Ciências Médicas da Santa Casa de São Paulo, São Paulo (SP), Brasil.
. Grupo Fleury, Pesquisa e Desenvolvimento, São Paulo (SP), Brasil.
J Bras Pneumol. 2023 Feb 6;49(1):e20220040. doi: 10.36416/1806-3756/e20220040. eCollection 2023.
The aim of this study was to assess the laboratory performance of periostin associated with a panel of biomarkers to identify the inflammatory phenotype of Brazilian asthma patients.
We evaluated 103 Brazilian individuals, including 37 asthmatics and 66 nonasthmatic controls. Both groups underwent analyses for serum periostin, eosinophil levels in the peripheral blood, the fraction of exhaled nitric oxide (FeNO), total serum IgE, urinary leukotriene E4, and serum cytokines.
Higher levels of periostin (p = 0.005), blood eosinophils (p = 0.012), FeNO (p = 0.001), total IgE (p < 0.001), and IL-6 (p ≤ 0.001) were found in the asthmatic patients than the controls. Biomarker analyses by the ROC curve showed an AUC greater than 65%. Periostin (OR: 12,550; 95% CI: 2,498-63,063) and IL-6 (OR: 7,249; 95% CI: 1,737-30,262) revealed to be suitable asthma inflammation biomarkers. Blood eosinophils, FeNO, total IgE, IL-6, TNF, and IFN-g showed correlations with clinical severity characteristics in asthmatic patients. Periostin showed higher values in T2 asthma (p = 0.006) and TNF in non-T2 asthma (p = 0.029).
The panel of biomarkers proposed for the identification of the inflammatory phenotype of asthmatic patients demonstrated good performance. Periostin proved to be an important biomarker for the identification of T2 asthma.
本研究旨在评估与一组生物标志物相关的骨膜蛋白的实验室表现，以确定巴西哮喘患者的炎症表型。
我们评估了 103 名巴西个体，包括 37 名哮喘患者和 66 名非哮喘对照者。两组均进行血清骨膜蛋白、外周血嗜酸性粒细胞水平、呼气一氧化氮分数（FeNO）、总血清 IgE、尿白三烯 E4 和血清细胞因子分析。
哮喘患者的骨膜蛋白（p = 0.005）、血嗜酸性粒细胞（p = 0.012）、FeNO（p = 0.001）、总 IgE（p < 0.001）和 IL-6（p ≤ 0.001）水平均高于对照组。ROC 曲线的生物标志物分析显示 AUC 大于 65%。骨膜蛋白（OR：12550；95%CI：2498-63063）和 IL-6（OR：7249；95%CI：1737-30262）显示为适合的哮喘炎症生物标志物。血嗜酸性粒细胞、FeNO、总 IgE、IL-6、TNF 和 IFN-γ与哮喘患者的临床严重程度特征相关。T2 哮喘患者的骨膜蛋白（p = 0.006）和非 T2 哮喘患者的 TNF（p = 0.029）值较高。
用于识别哮喘患者炎症表型的生物标志物组合表现良好。骨膜蛋白被证明是识别 T2 哮喘的重要生物标志物。
