Laboratory of Pathogen Microbiology and Immunology, College of Life Science, Jilin Agricultural University, Jilin Province, People's Republic of China.
Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Changchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Jilin Province, People's Republic of China.
J Vet Med Sci. 2023 Mar 28;85(3):393-398. doi: 10.1292/jvms.22-0441. Epub 2023 Mar 9.
Lymphocytic choriomeningitis virus (LCMV) is a neglected rodent-borne zoonotic virus in the genus Mammarenavirus and family Arenaviridae, that can cause aseptic meningitis in humans. A recent study identified infectious LCMV in ticks in northeastern (NE) China. To explore the distribution of LCMV, we determined the prevalence and genetically characterized LCMV in ticks in Jilin Province, NE China. Ticks collected in Huadian, Dunhua, and Jiaohe were pooled and LCMV was detected using reverse transcription polymerase chain reaction. The complete genomes of the LCMV-positive pools were amplified and used for phylogenetic analysis. A total of 1679 questing and engorged ticks were collected and divided into 170 pools, including Ixodes persulcatus (5%), Dermacentor silvarum (89%), and Haemaphysalis japonica (6%). Twenty-four pools of D. silvarum (14.9%, 95% CI:9.5-22.3) and three pools of H. japonica (36.3%, 95% CI:9.8-99.5) collected from cattle were LCMV-positive. No I. persulcatus pools were identified as LCMV-positive. Two complete genome sequences (strains JL-DH01 and JL-DH02) were successfully amplified. They had nucleotide identities of 96.4-99.8% with strains JX31, JX14, DH46, and JX4 identified in ticks from Jilin Province. Phylogenetic analysis indicated that JL-DH01 and JL-DH02 clustered with Jilin strains in the same branch and belonged to genotype I. The findings add to the knowledge of the genetic diversity and geographical distribution of LCMV in ticks in NE China.
淋巴细胞性脉络丛脑膜炎病毒（Lymphocytic choriomeningitis virus，LCMV）是一种被忽视的啮齿动物传播的人畜共患病病毒，属于 Mammarenavirus 属和 Arenaviridae 科，可导致人类无菌性脑膜炎。最近的一项研究在东北地区（NE）中国的蜱中发现了传染性 LCMV。为了探索 LCMV 的分布，我们确定了东北地区吉林省蜱中的 LCMV 流行率和遗传特征。从桦甸、敦化和蛟河收集的蜱被混合在一起，并使用逆转录聚合酶链反应检测 LCMV。阳性池的 LCMV 全长基因组被扩增并用于系统发育分析。共收集并分为 170 个池的 1679 只游离和吸血蜱，包括 Ixodes persulcatus（5%）、Dermacentor silvarum（89%）和 Haemaphysalis japonica（6%）。从牛中采集的 24 个 D. silvarum 池（14.9%，95%CI：9.5-22.3）和 3 个 H. japonica 池（36.3%，95%CI：9.8-99.5）为 LCMV 阳性。未鉴定出 I. persulcatus 池为 LCMV 阳性。成功扩增了两个全长基因组序列（菌株 JL-DH01 和 JL-DH02）。它们与从吉林省蜱中鉴定的菌株 JX31、JX14、DH46 和 JX4 的核苷酸同一性为 96.4-99.8%。系统发育分析表明，JL-DH01 和 JL-DH02 与吉林株在同一分支聚类，属于基因型 I。研究结果增加了 LCMV 在东北地区蜱中的遗传多样性和地理分布的知识。
