鼻黏膜组织中白细胞特异性转录物 1 水平对预测鼻息肉复发的意义。
Significance of leukocyte-specific transcript 1 levels in nasal mucosal tissue to predict recurrence of nasal polyps.
机构信息
Xiangya Hospital of Central South University, Department of Otolaryngology-Head and Neck Surgery, Changsha, Hunan, China.
Xiangya Hospital of Central South University, Department of Otolaryngology-Head and Neck Surgery, Changsha, Hunan, China; Hunan Province Key Laboratory of Otolaryngology Critical Diseases, Changsha, Hunan, China; National Clinical Research Center for Geriatric Disorders, Changsha, Hunan, China.
出版信息
Braz J Otorhinolaryngol. 2023 Mar-Apr;89(2):321-328. doi: 10.1016/j.bjorl.2022.11.004. Epub 2022 Dec 12.
OBJECTIVE
Chronic Rhinosinusitis with Polyps (CRSwNP) is characterized by high heterogeneity and postoperative recurrence rate. This study aims to explore the clinical significance of tissue Leukocyte-Specific Transcript 1 (LST1) in predicting CRSwNP recurrence.
METHODS
We enrolled 62 CRSwNP patients including 30 primary CRSwNP and 32 recurrent CRSwNP patients, and 40 Healthy Controls (HC). Tissue samples were collected. Tissue LST1 expression was assessed by Reverse Transcription-Polymerase Chain Reaction (RT-PCR), Western Blotting (WB) and Immunofluorescence (IF) staining. The predictive values of LST1 expression for CRSwNP postoperative recurrence were assessed through the Receiver Operating Characteristic (ROC) curves.
RESULTS
The tissue levels of LST1 were significantly increased in the CRSwNP group than the HC group, especially in the recurrent group, and the elevated LST1 mRNA levels were positively correlated with the peripheral eosinophil percentages, tissue eosinophil counts and percentages. IF staining results showed that the LST1 protein levels were higher in CRSwNP patients, especially in the recurrent patients than in the HC group. ROC curves highlighted that tissue LST1 levels were associated with recurrent CRSwNP and exhibited a higher predictive ability for postoperative CRSwNP recurrence.
CONCLUSION
This was the first report suggesting that LST1 expression was upregulated and associated with mucosal eosinophil infiltration and CRSwNP recurrence. Tissue LST1 could be a promising biomarker for predicting postoperative recurrence in CRwNP patients.
LEVEL OF EVIDENCE
Level 5.
目的
伴有鼻息肉的慢性鼻-鼻窦炎（CRSwNP）的特点是具有高度异质性和术后复发率高。本研究旨在探讨组织白细胞特异性转录物 1（LST1）在预测 CRSwNP 复发中的临床意义。
方法
我们纳入了 62 例 CRSwNP 患者，包括 30 例初发 CRSwNP 和 32 例复发 CRSwNP 患者，以及 40 例健康对照（HC）。收集组织样本。通过逆转录-聚合酶链反应（RT-PCR）、Western Blotting（WB）和免疫荧光（IF）染色评估组织 LST1 表达。通过受试者工作特征（ROC）曲线评估 LST1 表达对 CRSwNP 术后复发的预测价值。
结果
CRSwNP 组的组织 LST1 水平明显高于 HC 组，尤其是在复发组，升高的 LST1 mRNA 水平与外周嗜酸性粒细胞百分比、组织嗜酸性粒细胞计数和百分比呈正相关。IF 染色结果显示，CRSwNP 患者的 LST1 蛋白水平较高，尤其是在复发患者中高于 HC 组。ROC 曲线强调，组织 LST1 水平与复发性 CRSwNP 相关，对术后 CRSwNP 复发具有较高的预测能力。
结论
这是首个报告表明 LST1 表达上调，并与鼻黏膜嗜酸性粒细胞浸润和 CRSwNP 复发相关。组织 LST1 可能是预测 CRSwNP 患者术后复发的有前途的生物标志物。
证据水平
5 级。
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