Department of Otolaryngology, Head and Neck Surgery, Beijing TongRen Hospital, Capital Medical University, Beijing, P.R. China; Beijing Key Laboratory of Nasal Diseases, Beijing Institute of Otolaryngology, Beijing, P.R. China.
Department of Otolaryngology, Head and Neck Surgery, Beijing TongRen Hospital, Capital Medical University, Beijing, P.R. China; Beijing Key Laboratory of Nasal Diseases, Beijing Institute of Otolaryngology, Beijing, P.R. China; Department of Allergy, Beijing TongRen Hospital, Capital Medical University, Beijing, P.R. China.
Rhinology. 2020 Apr 1;58(2):166-174. doi: 10.4193/Rhin19.296.
Tissue eosinophils have been shown to be associated with polyp recurrence in chronic rhinosinusitis with nasal polyps (CRSwNP). We addressed whether the mRNA levels of Charcot-Leyden Crystal (CLC) in nasal brushing samples, a molecule mainly released from activated eosinophils, could serve as an effective non-invasive biomarker to predict polyp recurrence.
A total of 51 patients with CRSwNP completing the postoperative follow-up over a period of 12-18 months were enrolled. Baseline CLC mRNA levels of the nasal brushings collected prior to endoscopic sinus surgery were quantified by quantitative real-time polymerase chain reaction (qRT-PCR). Polyp specimens were collected during surgery and were evaluated for inflammatory cells by histopathologic staining. The patients' baseline characteristics were reviewed and analyzed for associations with recurrence. Logistic regression analysis was performed to determine the predictive factors for polyp recurrence, and receiver operating characteristic (ROC) curves were performed to determine their predictive values.
Overall, 25/51(49.02%) patients experienced polyp recurrence during the 12-18 months follow-up. The baseline relative CLC mRNA level in nasal brushing samples was significantly increased in patients with recurrence compared to those without recurrence (p.
组织嗜酸性粒细胞与慢性鼻-鼻窦炎伴鼻息肉（CRSwNP）患者的息肉复发有关。我们研究了鼻刷样本中 Ch arcot-Leyden 晶体（CLC）mRNA 水平是否可以作为预测息肉复发的有效非侵入性生物标志物，CLC 是一种主要从活化的嗜酸性粒细胞中释放的分子。
共纳入 51 例在术后 12-18 个月完成随访的 CRSwNP 患者。通过实时定量聚合酶链反应（qRT-PCR）对内镜鼻窦手术前鼻刷采集的 CLC mRNA 水平进行定量。在手术过程中收集息肉标本，并通过组织病理学染色评估炎症细胞。回顾和分析患者的基线特征与复发的关系。进行逻辑回归分析以确定息肉复发的预测因素，并绘制受试者工作特征（ROC）曲线以确定其预测值。
总体而言，51 例患者中有 25 例（49.02%）在 12-18 个月的随访中出现息肉复发。与无复发患者相比，复发患者的鼻刷样本中 CLC mRNA 水平明显升高（p<0.001）。多因素逻辑回归分析显示，基线 CLC mRNA 水平与息肉复发独立相关（优势比 1.426，95%置信区间 1.058-1.918，p=0.021）。ROC 曲线分析显示，基于基线 CLC mRNA 水平预测息肉复发的曲线下面积为 0.68（95%置信区间 0.58-0.77，p<0.001），最佳截断值为 1.15（敏感性 71.3%，特异性 66.7%）。
鼻刷样本中 CLC mRNA 水平可能是预测 CRSwNP 患者术后息肉复发的有效非侵入性生物标志物。
