有丝分裂原刺激可短暂增加大鼠胸腺细胞中可交换的线粒体钙池。
Mitogenic stimulation transiently increases the exchangeable mitochondrial calcium pool in rat thymocytes.
作者信息
Lakin-Thomas P L, Brand M D
机构信息
Department of Biochemistry, University of Cambridge, U.K.
出版信息
Biochem J. 1987 Aug 15;246(1):173-7. doi: 10.1042/bj2460173.
Abstract
Exchangeable calcium pools were measured in rat thymocytes by 45Ca labelling and selective depletion of intracellular pools with oligomycin in the presence or absence of rotenone. The mitochondrial pool increased by 150% after 3 min of treatment with the mitogen concanavalin A, and decreased to zero 10 min after mitogen addition. No significant change in the ATP-dependent pool could be detected.
摘要
通过用⁴⁵Ca标记以及在存在或不存在鱼藤酮的情况下用寡霉素选择性耗尽细胞内钙库，来测定大鼠胸腺细胞中的可交换钙库。在用促细胞分裂剂伴刀豆球蛋白A处理3分钟后，线粒体钙库增加了150%，在添加促细胞分裂剂10分钟后降至零。未检测到ATP依赖性钙库有显著变化。
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