猪淋巴细胞中促细胞分裂剂激活丙酮酸脱氢酶的机制。
Mechanism of activation of pyruvate dehydrogenase by mitogens in pig lymphocytes.
作者信息
Baumgarten E, Brand M D, Pozzan T
出版信息
Biochem J. 1983 Nov 15;216(2):359-67. doi: 10.1042/bj2160359.
Abstract
The activity of pyruvate dehydrogenase in extracts of pig mesenteric lymphocytes was measured under different preincubation conditions. The mitogens concanavalin A and ionophore A23187 both increased pyruvate dehydrogenase activity. In both cases activation required extracellular Ca2+. Digitonin-permeabilized cells required 0.5 microM free Ca2+ for half-maximal activation of pyruvate dehydrogenase. The stimulation by concanavalin A in intact cells was probably not due to changes in effectors of pyruvate dehydrogenase kinase. This evidence suggests that activation of pyruvate dehydrogenase is by Ca2+ activation of pyruvate dehydrogenase phosphatase and supports the view that the cytoplasmic free [Ca2+] rises to something less than 1 microM on stimulation with mitogens.
摘要
在不同的预孵育条件下，测定了猪肠系膜淋巴细胞提取物中丙酮酸脱氢酶的活性。促有丝分裂原伴刀豆球蛋白A和离子载体A23187均能提高丙酮酸脱氢酶的活性。在这两种情况下，激活都需要细胞外Ca2+。用洋地黄皂苷通透处理的细胞，丙酮酸脱氢酶半最大激活需要0.5微摩尔游离Ca2+。在完整细胞中，伴刀豆球蛋白A的刺激作用可能不是由于丙酮酸脱氢酶激酶的效应物发生了变化。这一证据表明，丙酮酸脱氢酶的激活是通过丙酮酸脱氢酶磷酸酶的Ca2+激活实现的，支持了在有丝分裂原刺激下细胞质游离[Ca2+]升高至小于1微摩尔的观点。
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Activation of lymphocyte glycogen phosphorylase by mitogens.丝裂原对淋巴细胞糖原磷酸化酶的激活作用。
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Evidence that insulin activates an intrinsic plasma membrane protease in generating a secondary chemical mediator.有证据表明胰岛素在生成次级化学介质时会激活一种内在质膜蛋白酶。
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Calcium homeostasis in intact lymphocytes: cytoplasmic free calcium monitored with a new, intracellularly trapped fluorescent indicator.完整淋巴细胞中的钙稳态：用一种新的细胞内捕获荧光指示剂监测细胞质游离钙。
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Lectins activate lymphocyte pyruvate dehydrogenase by a mechanism sensitive to protease inhibitors.凝集素通过一种对蛋白酶抑制剂敏感的机制激活淋巴细胞丙酮酸脱氢酶。
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T-cell mitogens cause early changes in cytoplasmic free Ca2+ and membrane potential in lymphocytes.T细胞有丝分裂原可引起淋巴细胞胞质游离Ca2+和膜电位的早期变化。
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