Pan Shijun, Wang Gang, Fan Yide, Wang Xiqing, Liu Juan, Guo Mingzhu, Chen Huan, Zhang Sitong, Chen Guang
College of Life Science, Jilin Agricultural University, Jilin, 130118, China.
Key Laboratory of Straw Comprehensive Utilization and Black Land Conservation, Education Ministry of China, Jilin Agricultural University, Jilin, 130118, China.
Heliyon. 2023 Jan 27;9(2):e13246. doi: 10.1016/j.heliyon.2023.e13246. eCollection 2023 Feb.
In this paper, the relationship between black liquor and microbial growth, enzymatic secretion and humus formation in composting was studied. The results showed that black liquor inoculation is an effective way to promote fermentation process. After black liquor inoculation, the abundance of , , and , which have the catalase and oxidase activity, has been significantly increased. The enzymatic activity of alkaline phosphatase, catalase, peroxidase and invertase was 40 mg/(g·24h), 6.5 mg/(g·20 min), 13 100 mg/(g·24h), and 6100 mg/(g·24h) respectively at day 18. Humic acid and fulvic acid concentration was 12 g/kg and 11 g/kg which is higher than that of the treatments of no black liquor inoculation. The results suggested that black liquor inoculation was beneficial to indigenous microorganisms reproduce efficiently, then the secretion of enzymes related to cellulose, hemicellulose, and lipid hydrolysis, and the formation of humic substances.
本文研究了黑液与堆肥过程中微生物生长、酶分泌及腐殖质形成之间的关系。结果表明,接种黑液是促进发酵过程的有效途径。接种黑液后,具有过氧化氢酶和氧化酶活性的 、 、 和 的丰度显著增加。在第18天,碱性磷酸酶、过氧化氢酶、过氧化物酶和转化酶的酶活性分别为40 mg/(g·24h)、6.5 mg/(g·20 min)、13 100 mg/(g·24h)和6100 mg/(g·24h)。腐植酸和富里酸浓度分别为12 g/kg和11 g/kg,高于未接种黑液处理。结果表明,接种黑液有利于土著微生物的高效繁殖,进而有利于纤维素、半纤维素和脂质水解相关酶的分泌以及腐殖质的形成。