Belen'kiĭ B G, Gankina E S, Kever E E, Kostiuk I O, Saminskiĭ A E
Bioorg Khim. 1987 Jul;13(7):894-7.
A new method of determination of molecular mass of proteins and peptides has been developed, basing on the microcolumn exclusion chromatography on non-modified silica sorbents in trifluoroacetic acid. It is shown that in this eluent the proteins and peptides adopt the random-coil conformation and are not hydrolyzed for three days at room temperature.
一种基于在三氟乙酸中使用未改性硅胶吸附剂的微柱排阻色谱法测定蛋白质和肽分子量的新方法已经开发出来。结果表明，在这种洗脱液中，蛋白质和肽呈现无规卷曲构象，并且在室温下三天内不会被水解。
