Kever E E, Zimina T M, Belen'kiĭ B G
Bioorg Khim. 1989 Aug;15(8):1019-21.
A method of determination of molecular masses of globular proteins based on microcolumn size-exclusion HPLC on modified silica sorbent (TSK-GEL 3000 SW) with refractometric detection has been developed. Molecular masses in the range 10 to 200 kD were determined with the accuracy of +/- 3%.
基于配备折光检测的改性硅胶吸附剂(TSK-GEL 3000 SW)上的微柱尺寸排阻高效液相色谱法,开发了一种测定球状蛋白质分子量的方法。测定了10至200 kD范围内的分子量,准确度为±3%。