环状 RNA 0024108 促进食管癌细胞的进展。
Circ_0024108 promotes the progression of esophageal cancer cells.
机构信息
Department of Thoracic Surgery, The Affiliated Hospital of Southwest Medical University, No. 25 TaiPing St, Jiangyang District, Luzhou, 646000, Sichuan, People's Republic of China.
Department of Thoracic Surgery, The People's Hospital of Leshan, Leshan, 614000, Sichuan, China.
出版信息
Gen Thorac Cardiovasc Surg. 2023 Jul;71(7):418-431. doi: 10.1007/s11748-023-01909-8. Epub 2023 Feb 9.
BACKGROUND
Esophageal squamous cell carcinoma (ESCC) is a serious malignant cancer. The treatment effect of ESCC is relatively poor and needs further improvement. According to reports, circular RNAs (circRNAs) actively participate in human carcinogenesis. More explorations are needed about the action of circRNAs in ESCC.
METHODS
Circ_0024108, miR-488-3p, and USP14 was quantified by a qRT-PCR or immunoblotting method. Cell proliferation evaluation was performed by MTT, EdU, and colony formation assays. Evaluation of cell motility and invasiveness was conducted using wound healing assay and transwell assay. The regulatory mechanism of circ_0024108, miR-488-3p, and USP14 was detected by RNA pull-down assay and dual-luciferase reporter assay.
RESULTS
Circ_0024108 and USP14 were significantly overexpressed in ESCC, while miR-488-3p was underexpressed. Deficiency of circ_0024108 impeded cell growth, motility, and invasiveness. Circ_0024108 regulated the expression of USP14 in ESCC cells via miR-488-3p. Also, circ_0024108 was present at high levels in serum exosomes from ESCC patients with high specificity and sensitivity.
CONCLUSIONS
Taken together, circ_0024108 participated in the progress of ESCC through the miR-488-3p/USP14 axis. Circ_0024108 was differentially expressed in serum exosomes. Circ_0024108 might be a potential biomarker for the diagnosis of ESCC.
背景
食管鳞状细胞癌（ESCC）是一种严重的恶性肿瘤。ESCC 的治疗效果相对较差，需要进一步改善。据报道，环状 RNA（circRNA）积极参与人类癌变。需要进一步探索 circRNA 在 ESCC 中的作用。
方法
通过 qRT-PCR 或免疫印迹法定量检测 circ_0024108、miR-488-3p 和 USP14。通过 MTT、EdU 和集落形成测定评估细胞增殖。通过划痕愈合试验和 Transwell 试验评估细胞迁移和侵袭能力。通过 RNA 下拉测定和双荧光素酶报告测定检测 circ_0024108、miR-488-3p 和 USP14 的调控机制。
结果
circ_0024108 和 USP14 在 ESCC 中显著过表达，而 miR-488-3p 表达下调。circ_0024108 缺失抑制了细胞生长、迁移和侵袭。circ_0024108 通过 miR-488-3p 调节 ESCC 细胞中 USP14 的表达。此外，circ_0024108 在 ESCC 患者血清外泌体中高水平表达，具有高特异性和灵敏度。
结论
综上所述，circ_0024108 通过 miR-488-3p/USP14 轴参与 ESCC 的进展。circ_0024108 在血清外泌体中差异表达。circ_0024108 可能是 ESCC 诊断的潜在生物标志物。
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