Jahan Kifat, Nie Hongtao, Yan Xiwu
College of Fisheries and Life Science, Dalian Ocean University, 116023, Dalian, China; Engineering Research Center of Shellfish Culture and Breeding in Liaoning Province, Dalian Ocean University, 116023, Dalian, China.
College of Fisheries and Life Science, Dalian Ocean University, 116023, Dalian, China; Engineering Research Center of Shellfish Culture and Breeding in Liaoning Province, Dalian Ocean University, 116023, Dalian, China.
Fish Shellfish Immunol. 2023 Mar;134:108607. doi: 10.1016/j.fsi.2023.108607. Epub 2023 Feb 8.
Heat Shock Protein (HSPs) gene family members play fundamental roles in different environmental stress tolerances, protect the structure and function of cells, and perform a significant task in cellular homeostasis. In this study, we conducted a genome-wide identification, evolutionary relationship analysis and gene expression analysis of the HSP70, HSP90, and HSF gene families in Ruditapes philippinarum. We identified 83 RpHSP70, 6 RpHSP90, and 3 RpHSF genes in R. philippinarum. The structural characteristics, chromosomal localization, and the gene structure map were constructed to reveal the characteristics of protein structures. Furthermore, the expression profiling of transcriptome data showed the expression pattern of HSP70, HSP90 and HSF genes in Manila clam from different populations, and under high and low temperature stress. In addition, we performed protein-protein interaction network analysis between HSP70, HSP90, and HSF gene family which enabled us to recognize the regulatory relationship between the two HSP gene families and the HSF gene family. Furthermore, the predicted sub-cellular location revealed a diversified subcellular distribution of HSP70, HSP90, and HSF proteins, which may be directly or indirectly associated with functional diversification under heat stress condition.
热休克蛋白（HSPs）基因家族成员在不同的环境胁迫耐受性中发挥着重要作用，保护细胞的结构和功能，并在细胞内稳态中执行重要任务。在本研究中，我们对菲律宾蛤仔的HSP70、HSP90和HSF基因家族进行了全基因组鉴定、进化关系分析和基因表达分析。我们在菲律宾蛤仔中鉴定出83个RpHSP70、6个RpHSP90和3个RpHSF基因。构建了其结构特征、染色体定位和基因结构图，以揭示蛋白质结构的特征。此外，转录组数据的表达谱显示了HSP70、HSP90和HSF基因在不同种群的菲律宾蛤仔中以及在高温和低温胁迫下的表达模式。此外，我们对HSP70、HSP90和HSF基因家族进行了蛋白质-蛋白质相互作用网络分析，这使我们能够识别两个HSP基因家族与HSF基因家族之间的调控关系。此外，预测的亚细胞定位揭示了HSP70、HSP90和HSF蛋白的亚细胞分布多样化，这可能与热应激条件下的功能多样化直接或间接相关。
