Li Dongpo, Yuan Yuan, Zhou Jinglong, Feng Zili, Wei Feng, Zhu Heqin, Zhao Lihong, Feng Hongjie, Zhang Yalin
State Key Laboratory of Cotton Bio-breeding and Integrated Utilization, Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang, Henan, China.
Western Agricultural Research Center of Chinese Academy of Agricultural Sciences, Changji, Xinjiang, China.
Front Microbiol. 2025 May 26;16:1535187. doi: 10.3389/fmicb.2025.1535187. eCollection 2025.
AHA1 (activator of HSP90 ATPase) is a co-chaperone protein that mainly performs its function by interacting with the HSP90. The biological function of AHA1 has been widely reported in many species. In this study, we knocked out the gene of by homologous recombination method. The knockout mutants showed increased drug sensitivity to ergosterol synthesis pathway, significantly inhibiting ergosterol biosynthesis. The knockout mutant strain also showed decreased melanin in microsclerotia by reduced expression of microsclerotia and melanin related genes , and . The mutant showed decreased conidial production that were slightly damaged and showed more sensitivity to abiotic stresses such as temperature, SDS, CR, Sorbitol (SBT), NaCl, and KCl and decreased ATP contents. More importantly, the mutant was significantly less virulent to cotton than the wild type. This study identified the important functions of in the growth, stress resistance, and virulence.
AHA1（热休克蛋白90 ATP酶激活剂）是一种共伴侣蛋白，主要通过与热休克蛋白90相互作用来发挥其功能。AHA1的生物学功能在许多物种中已被广泛报道。在本研究中，我们通过同源重组方法敲除了 基因。该 敲除突变体对麦角固醇合成途径表现出增加的药物敏感性，显著抑制了麦角固醇的生物合成。该 敲除突变体菌株还通过降低菌核和黑色素相关基因 和 的表达，使菌核中的黑色素减少。该 突变体显示分生孢子产量下降，分生孢子略有受损，并且对温度、SDS、CR、山梨醇（SBT）、NaCl和KCl等非生物胁迫表现出更高的敏感性，ATP含量降低。更重要的是，该突变体对棉花的毒力明显低于野生型。本研究确定了 在生长、抗逆性和毒力方面的重要功能。
