Tomza-Marciniak Agnieszka, Pilarczyk Bogumiła, Drozd Radosław, Pilarczyk Renata, Juszczak-Czasnojć Marta, Havryliak Viktoriia, Podlasińska Joanna, Udała Jan
Department of Animal Reproduction Biotechnology and Environmental Hygiene, West Pomeranian University of Technology, Szczecin, Janickiego 29, 71-270, Szczecin, Poland.
Department of Animal Reproduction Biotechnology and Environmental Hygiene, West Pomeranian University of Technology, Szczecin, Janickiego 29, 71-270, Szczecin, Poland.
Environ Pollut. 2023 Apr 1;322:121234. doi: 10.1016/j.envpol.2023.121234. Epub 2023 Feb 7.
The aim of this study was to a) evaluate the concentration of Se and Hg and their relationship in the tissues of 4 species of wild mammals, including Se:Hg molar ratios, and b) evaluate the effect of the analysed elements and their mutual proportions expressed as Se:Hg molar ratio, on the antioxidant system in the tissues of the tested animals. The study was performed on 31 animals belonging to four species: wild boar, red fox, roe deer, brown hare. Determination of Hg in liver, kidney and muscle of animals was performed using an AMA 254 mercury analyser. Total Se concentrations were determined using the spectrofluorometric method. In omnivores demonstrated higher Se concentrations in all analysed organs compared to the herbivores. The highest concentration of Hg was found in the kidneys of the tested animals, and the lowest in the muscles. High and moderate correlation between Se and Hg was observed in the liver of omnivorous, while in herbivores this correlation was weak. In all analysed samples, the Se:Hg molar ratios were above 1 (min: liver 5.9, max: kidney 110). Generally, the highest Se:Hg ratio values were found in kidney and the lowest in liver of tested animals. No significant correlation was found between GPx, GST and SOD activity and Se or Hg concentration in analysed organs. But it was observed that Se:Hg molar was negatively correlated with CAT activity in the most samples. The obtained results may suggest that omnivorous animals demonstrate greater Hg sequestration in the liver than herbivores, which has been proposed as one of the mechanisms of Se antagonistic action towards Hg. The ratio between Se and Hg, rather than the concentration of these elements in organs, affected the antioxidant status in the animal organism, specifically the CAT activity.
a）评估4种野生哺乳动物组织中硒（Se）和汞（Hg）的浓度及其关系，包括Se:Hg摩尔比；b）评估所分析元素及其以Se:Hg摩尔比表示的相互比例对受试动物组织中抗氧化系统的影响。该研究对属于4个物种的31只动物进行：野猪、赤狐、狍、草兔。使用AMA 254汞分析仪测定动物肝脏、肾脏和肌肉中的汞含量。总硒浓度采用荧光分光光度法测定。结果表明，杂食动物所有分析器官中的硒浓度均高于食草动物。受试动物肾脏中汞的浓度最高，肌肉中最低。杂食动物肝脏中硒和汞之间存在高度和中度相关性，而在食草动物中这种相关性较弱。在所有分析样本中，Se:Hg摩尔比均高于1（最小值：肝脏5.9，最大值：肾脏110）。一般来说，受试动物肾脏中的Se:Hg比值最高，肝脏中最低。在所分析器官中，谷胱甘肽过氧化物酶（GPx）、谷胱甘肽S-转移酶（GST）和超氧化物歧化酶（SOD）活性与硒或汞浓度之间未发现显著相关性。但观察到，在大多数样本中，Se:Hg摩尔比与过氧化氢酶（CAT）活性呈负相关。所得结果可能表明，杂食动物肝脏中汞的螯合作用比食草动物更强，这被认为是硒对汞拮抗作用的机制之一。硒和汞的比例，而非这些元素在器官中的浓度，影响了动物机体的抗氧化状态，特别是CAT活性。
