Kumpati J
Department of Pediatrics, University of Colorado School of Medicine, Denver 80262.
Biochem Med Metab Biol. 1987 Oct;38(2):170-81. doi: 10.1016/0885-4505(87)90077-6.
Phenylalanine or tryptophan entrapped in small unilamellar liposomes was used to transport Phe or Trp across the red blood cell membrane. The incorporation of Phe or Trp into RBCs via liposomes markedly inhibited and reversed the in vitro sickling of deoxy Hb S. Furthermore, normal and SS RBCs loaded with Phe or Trp did not exhibit significant change in osmotic fragility, mechanical fragility, autohemolysis, and glycolysis when compared to untreated RBCs. In addition, the oxygen affinity measured as the P50 and concentrations of 2,3-DPG and ATP were not affected by the incorporation of Phe or Trp into AA or SS RBCs. These results demonstrate that this liposomal transport system which transferred Phe and Trp into intact RBCs did not have any adverse effect on RBC metabolism and function, and may have therapeutic implications in the treatment of sickle cell disease.
包裹在小单层脂质体中的苯丙氨酸或色氨酸被用于将苯丙氨酸或色氨酸转运穿过红细胞膜。通过脂质体将苯丙氨酸或色氨酸掺入红细胞显著抑制并逆转了脱氧血红蛋白S的体外镰变。此外，与未处理的红细胞相比，装载有苯丙氨酸或色氨酸的正常和镰状细胞贫血症红细胞在渗透脆性、机械脆性、自身溶血和糖酵解方面没有表现出显著变化。另外，以P50衡量的氧亲和力以及2,3 - 二磷酸甘油酸和三磷酸腺苷的浓度不受将苯丙氨酸或色氨酸掺入正常或镰状细胞贫血症红细胞的影响。这些结果表明，这种将苯丙氨酸和色氨酸转运到完整红细胞中的脂质体转运系统对红细胞代谢和功能没有任何不利影响，并且可能对镰状细胞病的治疗具有治疗意义。
