Mansotra Ritika, Ali Tahir, Bhagat Nancy, Vakhlu Jyoti
Metagenomic Laboratory, School of Biotechnology, University of Jammu, Jammu, India.
Front Plant Sci. 2023 Jan 24;14:1074185. doi: 10.3389/fpls.2023.1074185. eCollection 2023.
has been reported to be the most devastating pathogen of L., a commercially significant crop that yields the saffron spice. However, most of the pathogen isolations have been done from the diseased tissue, mostly from rotten corms, but no study has been conducted on diseased saffron fields. To fill the knowledge gap, the current study was carried out with the intention of recording the diversity of cultivable fungus species from saffron fields and screening them for pathogenicity towards saffron. The three study locations in Jammu and Kashmir, Srinagar (Pampore), Kishtwar, and Ramban, yielded a total of 45 fungal isolates. The internal transcribed spacer (ITS) of rDNA was used for the molecular identification. ITS rDNA-based sequence analysis classified all the operational taxonomic units (OTUs) into two phyla-Ascomycota (88.88%) and Mucoromycota (11.11%). Moreover, (57.77%), (17.77%), (11.11%), (4.44%), (4.44%), (2.22%), and (2.22%) all had different total abundances at the genus level. It was discovered that the saffron fields in Srinagar have fewer varied fungal species than the other two selected sites. All of the fungal isolates isolated including , , , , and were pathogenic according to the pathogenicity test; however, injury to the saffron plant was found to be a must. These fungi were pathogenic in addition to , which is well documented as a major cause of saffron corm rot diseases in Srinagar, but in the present study, injury was a must for as well. The percentage disease severity index for both saffron roots and corms varied for each fungal isolate.
据报道,它是番红花(一种商业上重要的作物,可产出藏红花香料)最具毁灭性的病原体。然而,大多数病原体分离工作是从患病组织进行的,主要是从腐烂的球茎中分离,但尚未对患病的藏红花田进行研究。为填补这一知识空白,本研究旨在记录藏红花田中可培养真菌物种的多样性,并筛选它们对藏红花的致病性。查谟和克什米尔的三个研究地点,斯利那加(潘波雷)、基什特瓦尔和兰班,共获得了45株真菌分离株。rDNA的内部转录间隔区(ITS)用于分子鉴定。基于ITS rDNA的序列分析将所有操作分类单元(OTU)分为两个门——子囊菌门(88.88%)和毛霉门(11.11%)。此外,在属水平上,[具体属名1](57.77%)、[具体属名2](17.77%)、[具体属名3](11.11%)、[具体属名4](4.44%)、[具体属名5](4.44%)、[具体属名6](2.22%)和[具体属名7](2.22%)的总丰度各不相同。研究发现,斯利那加的藏红花田的真菌物种多样性比其他两个选定地点少。根据致病性测试,所有分离出的真菌分离株,包括[具体真菌1]、[具体真菌2]、[具体真菌3]、[具体真菌4]和[具体真菌5]都是致病的;然而,发现对藏红花植株造成损伤是必要条件。除了[具体真菌6]外,这些真菌也是致病的,[具体真菌6]是斯利那加藏红花球茎腐烂病的主要原因,这已得到充分记录,但在本研究中,对[具体真菌6]来说造成损伤也是必要条件。每种真菌分离株对藏红花根和球茎的病情严重指数百分比各不相同。